Université de Fribourg

A rational and iterative process for targeted nanoparticle design and validation

Rodriguez-Lorenzo, Laura ; D.Rafiee, Sarah ; Reis, Corine ; Milosevic, Ana ; L.Moore, Thomas ; Balog, Sandor ; Rothen-Rutishauser, Barbara ; Ruegg, Curzio ; Petri-Fink, Alke

In: Colloids and Surfaces B: Biointerfaces, 2018, vol. 171, p. 579–589

The lack of understanding of fundamental nano-bio interactions, and difficulties in designing particles stable in complex biological environments are major limitations to their translation into biomedical clinical applications. Here we present a multi- parametric approach to fully characterize targeted nanoparticles, and emphasizes the significant effect that each detail in the synthetic...

Université de Fribourg

PI3Kγ activity in leukocytes promotes adipose tissue inflammation and early-onset insulin resistance during obesity

Breasson, Ludovic ; Becattini, Barbara ; Zani, Claudia Sardi†, Angela Molinaro†, Fabio ; Marone, Romina ; Botindari, Fabrizio ; Bousquenaud, Mélanie ; Ruegg, Curzio ; Wymann, Matthias P. ; Solinas, Giovanni

In: Science Signaling, 2017, vol. 10, no. 488, p. eaaf2969

The phosphoinositide 3-kinase γ (PI3Kγ) plays a major role in leukocyte recruitment during acute inflammation and has been proposed to inhibit classical macrophage activation by driving immunosuppressive gene expression. PI3Kγ plays an important role in diet-induced obesity and insulin resistance. In seeking to determine the underlying molecular mechanisms, we showed that PI3Kγ action in...

Université de Fribourg

Computational modeling of shear forces and experimental validation of endothelial cell responses in an orbital well shaker system

Filipovic, Nenad ; Ghimire, Kedar ; Saveljic, Igor ; Milosevic, Zarko ; Ruegg, Curzio

In: Computer Methods in Biomechanics and Biomedical Engineering, 2016, vol. 19, no. 6, p. 581–590

Vascular endothelial cells are continuously exposed to hemodynamic shear stress. Intensity and type of shear stress are highly relevant to vascular physiology and pathology. Here, we modeled shear stress distribution in a tissue culture well (R = 17.5 mm, fill volume 2 ml) under orbital translation using computational fluid dynamics with the finite element method. Free surface distribution, wall...

Université de Fribourg

Discovery of a 29-gene panel in peripheral blood mononuclear cells for the detection of colorectal cancer and adenomas using high throughput real-time PCR

Ciarloni, Laura ; Hosseinian, Sahar ; Monnier-Benoit, Sylvain ; Imaizumi, Natsuko ; Dorta, Gian ; Ruegg, Curzio

In: PLoS ONE, 2015, vol. 10, no. 4, p. e0123904

Colorectal cancer (CRC) is the second leading cause of cancer-related death in developed countries. Early detection of CRC leads to decreased CRC mortality. A blood-based CRC screening test is highly desirable due to limited invasiveness and high acceptance rate among patients compared to currently used fecal occult blood testing and colonoscopy. Here we describe the discovery and validation of a...

Université de Fribourg

Molecular Pathways: Emerging pathways mediating growth, invasion and metastasis of tumors progressing in an irradiated microenvironment

Kuonen, Francois ; Secondini, Chiara ; Ruegg, Curzio

In: Clinical Cancer Research, 2012, p. -

Radiotherapy is a well-established therapeutic modality in oncology. It provides survival benefits in several different cancer types. However, cancers relapsing after radiotherapy often develop into more aggressive conditions, which are difficult to treat and are associated with poor prognosis. Cumulating experimental evidence indicates that the irradiated tumor bed contributes to such aggressive...

Université de Fribourg

Inhibition of the Kit ligand/c-Kit axis attenuates metastasis in a mouse model mimicking local breast cancer relapse after radiotherapy

Kuonen, Francois ; Laurent, Jullien ; Secondini, Chiara ; Lorusso, Girieca ; Stehle, Jean-Christophe ; Rausch, Thierry ; Hull, Eveline Faes-van't ; Bieler, Gregory ; Alghisi, Gian Carlo ; Schwendener, Reto A. ; Andrejevic-Blant, Snezana ; Mirimanoff, René-Olivier ; Ruegg, Curzio

In: Clinical Cancer Research, 2012, p. -

Purpose: Local breast cancer relapse after breast-saving surgery and radiotherapy is associated with increased risk of distant metastasis formation. The mechanisms involved remain largely elusive. We used the well-characterized 4T1 syngeneic, orthotopic breast cancer model to identify novel mechanisms of post-radiation metastasis. Experimental Design: 4T1 cells were injected in 20 Gy...

Université de Fribourg

Emerging paradigms and questions on pro-angiogenic bone marrow-derived myelomonocytic cells

Laurent, Julien ; Touvrey, Cédric ; Botta, Francesca ; Kuonen, François ; Ruegg, Curzio

In: The International Journal of Developmental Biology, 2011, vol. 55, p. 527-534

Cancer-related inflammation has emerged in recent years as a major event contributing to tumor angiogenesis, tumor progression and metastasis formation. Bone marrow-derived and inflammatory cells promote tumor angiogenesis by providing endothelial progenitor cells that differentiate into mature endothelial cells, and by secreting pro-angiogenic factors and remodeling the extracellular matrix to...

Université de Fribourg

Autologous stem cell transplantation: leukapheresis product has anti-angiogenic effects in vivo correlating with neutrophil-derived VEGFR1

Luethy, Anita ; Stenner, Frank ; Lohri, Corinne ; Muller, Christoph ; Samaras, Panagiotis ; Steiner, Rudolf ; Broek, Maries Van Den ; Mischo, Axel ; Renner, Christoph ; Knuth, Alexander ; Ruegg, Curzio ; Wenger, Roland H. ; Zweifel, Martin

In: Anticancer Research, 2011, vol. 31, no. 10, p. 3115-3124

Background: High-dose chemotherapy (HDC) followed by autologous stem cell transplantation (ASCT) is used for the treatment of hemato-oncologic malignancies. In this study, we measured the effect of HDC/ASCT on plasma concentrations of antiangiogenic soluble vascular endothelial growth factor receptor 1 (sVEGFR1) and of leukapheresis products (LP) and patient serum on chick chorioallantoic (CAM)...

Université de Fribourg

Proangiogenic factor PlGF programs CD11b+ myelomonocytes in breast cancer during differentiation of their hematopoietic progenitors

Laurent, Julien ; Faes-van't Hull, Eveline ; Touvrey, Cedric ; Kuonen, Francois ; Lan, Qiang ; Lorusso, Girieca ; Doucey, Marie-Agnes ; Ciarloni, Laura ; Imaizumi, Natsuko ; Alghisi, Gian Carlo ; Fagiani, Ernesta ; Zaman, Khalil ; Stupp, Roger ; Shibuya, Masabumi ; Delaloye, Jean-François ; Christofori, Gerhard ; Ruegg, Curzio

In: Cancer Research, 2011, p. -

Tumor-mobilized bone marrow-derived CD11b+ myeloid cells promote tumor angiogenesis, but how and when these cells acquire proangiogenic properties is not fully elucidated. Here we show that CD11b+ myelomonocytic cells develop proangiogenic properties during their differentiation from CD34+ hematopoietic progenitors and that Placenta Growth Factor (PlGF) is critical in promoting this education....