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Université de Fribourg

The calcium-binding protein parvalbumin modulates the firing 1 properties of the reticular thalamic nucleus bursting neurons

Albéri, Lavinia ; Lintas, Alessandra ; Kretz, Robert ; Schwaller, Beat ; Villa, Alessandro E. P.

In: Journal of Neurophysiology, 2013, vol. 109, no. 11, p. 2827–2841

The reticular thalamic nucleus (RTN) of the mouse is characterized by an overwhelming majority of GABAergic neurons receiving afferences from both the thalamus and the cerebral cortex and sending projections mainly on thalamocortical neurons. The RTN neurons express high levels of the “slow Ca²⁺ buffer” parvalbumin (PV) and are characterized by low-threshold Ca²⁺ currents,...

Université de Fribourg

Restricted diffusion of calretinin in cerebellar granule cell dendrites implies Ca²⁺-dependent interactions via its EF-hand 5 domain

Arendt, Oliver ; Schwaller, Beat ; Brown, Edward B. ; Eilers, Jens ; Schmidt, Hartmut

In: The Journal of Physiology, 2013, p. –

Ca²⁺-binding proteins (CaBPs) are important regulators of neuronal Ca²⁺ signaling, acting either as buffers that shape Ca²⁺ transients and Ca²⁺ diffusion and/or as Ca²⁺ sensors. The diffusional mobility represents a crucial functional parameter of CaBPs, describing their range-of-action and possible interactions with binding partners. Calretinin (CR) is a CaBP widely expressed in...

Université de Fribourg

Renal expression of parvalbumin is critical for NaCl handling and response to diuretics

Belge, Hendrica ; Gailly, Philippe ; Schwaller, Beat ; Loffing, Johannes ; Debaix, Huguette ; Riveira-Munoz, Eva ; Beauwens, Renaud ; Devogelaer, Jean-Pierre ; Hoenderop, Joost G. ; Bindels, René J. ; Devuyst, Olivier

In: Proceedings of the National Academy of Sciences of the United States of America, 2007, vol. 104, no. 37, p. 14849-14854

The distal convoluted tubule (DCT) plays an essential role in the reabsorption of NaCl by the kidney, a process that can be inhibited by thiazide diuretics. Parvalbumin (PV), a Ca²⁺-binding protein that plays a role in muscle fibers and neurons, is selectively expressed in the DCT, where its role remains unknown. We therefore investigated the renal phenotype of PV knockout mice...

Université de Fribourg

Establishment of immortalized murine mesothelial cells and a novel mesothelioma cell line

Blum, Walter ; Pecze, László ; Felley-Bosco, Emanuela ; Worthmüller-Rodriguez, Janine ; Wu, Licun ; Vrugt, Bart ; Perrot, Marc de ; Schwaller, Beat

In: In Vitro Cellular & Developmental Biology - Animal, 2015, p. 1–8

Mesothelial cells are susceptible to 10:38 05.06.2015asbestos fiber-induced cytotoxicity and on longer time scales to transformation; the resulting mesothelioma is a highly aggressive neoplasm that is considered as incurable at the present time Zucali et al. (Cancer Treatment Reviews 37:543–558, 2011). Only few murine cell culture models of immortalized mesothelial cells and mesothelioma...

Université de Fribourg

Regulation of calretinin in malignant mesothelioma is mediated by septin 7 binding to the CALB2 promoter

Blum, Walter ; Pecze, László ; Wörthmüller Rodriguez, Janine ; Steinauer, Martine ; Schwaller, Beat

In: BMC Cancer, 2018, vol. 18, p. 475

The calcium-binding protein calretinin (gene name: CALB2) is currently considered as the most sensitive and specific marker for the diagnosis of malignant mesothelioma (MM). MM is a very aggressive tumor strongly linked to asbestos exposure and with no existing cure so far. The mechanisms of calretinin regulation, as well as its distinct function in MM are still poorly understood.

Université de Fribourg

Absence of calretinin protein expression in malignant mesotheliomas from asbestos-exposed NF2+/− mice and mouse mesothelioma cell lines from various mouse strains

Blum, Walter ; Henzi, Thomas ; Châtel-Soulet, Hugues-Etienne ; Pecze, Laszlo ; Wörthmüller Rodriguez, Janine ; Vrugt, Bart ; Schwaller, Beat

In: Biomarker Research, 2018, vol. 6, no. 1, p. 19

Calretinin is the most widespread positive marker for the immunohistochemical identification of malignant mesothelioma (MM) and was proposed to serve as a blood- based biomarker. Functionally, evidence has accumulated that calretinin might be implicated in MM tumorigenesis. We aimed to identify calretinin (CR; Calb2) in murine MM and reactive mesothelial cells in granuloma from...

Université de Fribourg

Biological noise and positional effects influence cell stemness

Blum, Walter ; Henzi, Thomas ; Schwaller, Beat ; Pecze, László

In: Journal of Biological Chemistry, 2018, vol. 293, no. 14, p. 5247–5258

Biological (or cellular) noise is the random quantitative variability of proteins and other molecules in individual, genetically identical cells. As the result of biological noise in the levels of some transcription factors that determine a cell's differentiation status, differentiated cells may dedifferentiate to a stem cell state given a sufficiently long time period. Here, to provide...

Université de Fribourg

Stem cell factor-based identification and functional properties of in vitro-selected subpopulations of malignant mesothelioma cells

Blum, Walter ; Pecze, , László ; Felley-Bosco, Emanuela ; Wu, Licun ; Perrot, Marc de ; Schwaller, Beat

In: Stem Cell Reports, 2017, vol. 8, no. 4, p. 1005–1017

Malignant mesothelioma (MM) is an aggressive neoplasm characterized by a poor patient survival rate, because of rapid tumor recurrence following first-line therapy. Cancer stem cells (CSCs) are assumed to be responsible for initiating tumorigenesis and driving relapse after therapeutic interventions. CSC-enriched MM cell subpopulations were identified by an OCT4/SOX2 reporter approach and...

Université de Fribourg

Calretinin is essential for mesothelioma cell growth/survival in vitro: A potential new target for malignant mesothelioma therapy?

Blum, Walter ; Schwaller, Beat

In: International Journal of Cancer, 2013, p. –

Malignant mesothelioma (MM) are highly aggressive asbestos-related neoplasms, which show strong chemotherapy-resistance and there is no effective cure for MM so far. Calretinin (CR) is widely used as a diagnostic marker for epithelioid and mixed (biphasic) mesothelioma, but still little is known about CR's putative function(s) in tumorigenesis. CR protects against asbestos-induced acute...

Université de Fribourg

Nanodomain coupling between Ca2+ channels and Ca2+ sensors promotes fast and efficient transmitter release at a cortical GABAergic synapse

Bucurenciu, Iancu ; Kulik, Akos ; Schwaller, Beat ; Frotscher, Michael ; Jonas, Peter

In: Neuron, 2008, vol. 57, no. 4, p. 536-545

It is generally thought that transmitter release at mammalian central synapses is triggered by Ca²⁺ microdomains, implying loose coupling between presynaptic Ca²⁺ channels and Ca²⁺ sensors of exocytosis. Here we show that Ca²⁺ channel subunit immunoreactivity is highly concentrated in the active zone of GABAergic presynaptic terminals of putative parvalbumin-containing basket cells in...