Université de Fribourg

Acquisition of broad-spectrum cephalosporin resistance leading to colistin resistance in Klebsiella pneumoniae

Jayol, Aurélie ; Nordmann, Patrice ; Desroches, Marine ; Decousser, Jean-Winoc ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2016, vol. 60, no. 5, p. 3199–3201

An extended-spectrum β-lactamase (ESBL)-producing and colistin-resistant Klebsiella pneumoniae clinical isolate was recovered from a patient who was treated with cefotaxime. This isolate harbored a blaCTX-M-15 ESBL gene that was associated with an ISEcp1 insertion sequence. Transposition of that tandem occurred within the chromosomal mgrB gene, leading...

Université de Fribourg

Are animals a source of Stenotrophomonas maltophilia in human infections? Contributions of a nationwide molecular study

Jayol, Aurélie ; Corlouer, Camille ; Haenni, Marisa ; Darty, Mélanie ; Maillard, Karine ; Desroches, Marine ; Lamy, Brigitte ; Jumas-Bilak, Estelle ; Madec, Jean-Yves ; Decousser, Jean-Winoc

In: European Journal of Clinical Microbiology & Infectious Diseases, 2018, p. 1–7

Stenotrophomonas maltophilia (Sm) is an archetypal environmental opportunistic bacterium responsible for health care-associated infections. The role of animals in human Sm infections is unknown. This study aims to reveal the genetic and phylogenetic relationships between pathogenic strains of Sm, both animal and human, and identify a putative role for animals as a reservoir in human...

Université de Fribourg

Colistin resistance in Parisian inpatient faecal Escherichia coli as the result of two distinct evolutionary pathways

Bourrel, Anne Sophie ; Poirel, Laurent ; Royer, Guilhem ; Darty, Mélanie ; Vuillemin, Xavier ; Kieffer, Nicolas ; Clermont, Olivier ; Denamur, Erick ; Nordmann, Patrice ; Decousser, Jean-Winoc ; The IAME Resistance Group

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 6, p. 1521–1530

Beyond plasmid-encoded resistance (mcr genes) prevalence in strain collections, large epidemiological studies to estimate the human burden of colistin-resistant Escherichia coli gut carriage are lacking.Objectives: To evaluate the prevalence of colistin-resistant E. coli carriage in inpatients and decipher the molecular support of resistance and the genetic background of the strains.Methods:...

Université de Fribourg

mcr-9, An inducible gene encoding an acquired phosphoethanolamine transferase in Escherichia coli, and its origin

Kieffer, Nicolas ; Royer, b Guilhem ; Decousser, Jean-Winoc ; Bourrel, Anne-Sophie ; Palmieri, Mattia ; Ortiz De La Rosa, Jose-Manuel ; Jacquier, Hervé ; Denamur, Erick ; Nordmann, Patrice ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 9, p. -

The plasmid-located mcr-9 gene, encoding a putative phosphoethanolamine transferase, was identified in a colistin-resistant human fecal Escherichia coli strain belonging to a very rare phylogroup, the D-ST69-O15:H6 clone. This MCR-9 protein shares 33% to 65% identity with the other plasmid-encoded MCR-type enzymes identified (MCR-1 to -8) that have been found as sources of acquired resistance...

Université de Fribourg

Rapid polymyxin NP test for the detection of polymyxin resistance mediated by the MCR-1/MCR-2 genes

Poirel, Laurent ; Larpin, Yu ; Dobias, Jan ; Stephan, Roger ; Decousser, Jean-Winoc ; Madec, Jean-Yves ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 90, no. 1, p. 7–10

The Rapid Polymyxin NP test has been recently developed to rapidly detect polymyxin resistance in Enterobacteriaceae. Here we evaluated this test for detecting MCR- 1/MCR-2-producing Enterobacteriaceae using a collection of 70 non-redundant strains either recovered from the environment, animals, or humans. Sensitivity and specificity were found to be 100%.

Université de Fribourg

Recent advances in biochemical and molecular diagnostics for the rapid detection of antibiotic-resistant Enterobacteriaceae: a focus on ß-lactam resistance

Decousser, Jean-Winoc ; Poirel, Laurent ; Nordmann, Patrice

In: Expert Review of Molecular Diagnostics, 2017, vol. 17, no. 4, p. 327–350

The rapid detection of resistance is a challenge for clinical microbiologists who wish to prevent deleterious individual and collective consequences such as (i) delaying efficient antibiotic therapy, which worsens the survival rate of the most severely ill patients, or (ii) delaying the isolation of the carriers of multidrug-resistant bacteria and promoting outbreaks; this last consequence is...