Haute Ecole d'Ingénierie

Développement de la mobilité ionique pour la détection des sites de sumoylation des protéines = Identification of SUMOylation Sites using High-Field Asymmetric Waveform Ion Mobility (FAIMS) coupled to a Linear Ion Trap/Orbitrap Mass Spectrometer

Pfammatter, Sibylle ; Kalman, Franka (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2014.

SUMOylation is a post-translational modification of proteins that is involved in different cellular processes including mitosis, DNA replication and transcription. However its detection by liquid chromatography coupled to mass spectrometry (LC-MS) can be challenging due to its low abundance and dynamic regulation. High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) helps in...

Haute Ecole d'Ingénierie

Development of an analytical method usefull for the quantification of L-Glu & GlutaMAX during fermentation

Mayor, Mathieu ; Kalman, Franka (Dir.)

Mémoire de bachelor : Haute Ecole d'Ingénierie, 2013.

The objectives of this work are to establish time and cost-efficient methods for the quantification of L-Glutamine and GlutamaxTM in different mediums containing serums. These methods have to be easy to perform and based on a purpose of daily basis analysis of mammalian cells cultures.