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Université de Fribourg

Up-regulation of ryanodine receptor expression increases the calcium-induced calcium release and spontaneous calcium signals in cerebral arteries from hindlimb unloaded rats

Morel, Jean-Luc ; Dabertrand, Fabrice ; Porte3., Yves ; Prevot, Anne ; Macrez, Nathalie

In: Pflügers Archiv - European Journal of Physiology, 2014, vol. 466, no. 8, p. 1517–1528

Microgravity induces a redistribution of blood volume. Consequently, astronauts' body pressure is modified so that the upright blood pressure gradient is abolished, thereby inducing a modification in cerebral blood pressure. This effect is mimicked in the hindlimb unloaded rat model. After a duration of 8 days of unloading, Ca²⁺ signals activated by depolarization and...

Université de Fribourg

Rapid detection of extended-spectrum-β-lactamase-producing Enterobacteriaceae from urine samples by use of the ESBL NDP test

Dortet, Laurent ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2014, vol. 52, no. 10, p. 3701–3706

From June to September 2012, 500 urine samples were recovered from patients with urinary tract infections (UTI) due to Gram-negative bacilli (≥10⁴ leukocytes/ml and ≥10⁵ Gram-negative isolates/ml) who visited the University hospital Bicêtre (France). They were challenged with extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) using the rapid diagnostic ESBL NDP...

Université de Fribourg

Resistance to colistin associated with a single amino acid change in protein PmrB among Klebsiella pneumoniae isolates of worldwide Origin

Jayol, Aurélie ; Poirel, Laurent ; Brink, Adrian ; Villegas, Maria-Virginia ; Yilmaz, Mesut ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2014, vol. 58, no. 8, p. 4762–4766

A series of colistin-resistant Klebsiella pneumoniae isolates recovered from different countries was investigated in order to evaluate the involvement of the PmrA/PmrB two-component system in this resistance. Six isolates possessed a mutated PmrB protein, which is encoded by the pmrB gene, part of the pmrCAB operon involved in lipopolysaccharide modification. The same amino acid substitution...

Université de Fribourg

Multidrug-resistant Acinetobacter baumannii strains carrying the blaOxA-23 and the blaGES-11 genes in a neonatology center in Tunisia

Charfi-Kessis, Karama ; Mansour, Wejdene ; Khalifa, Anis Ben Haj ; Mastouri, Maha ; Nordmann, Patrice ; Aouni, Mahjoub ; Poirel, Laurent

In: Microbial Pathogenesis, 2014, vol. 74, p. 20–24

Multidrug-resistant and difficult-to-treat Acinetobacter baumannii may be responsible for nosocomial infections. The production of carbapenem-hydrolyzing class D β-lactamases (CHDLs) and extended-spectrum β-lactamase (ESBLs) of the GES type possessing a carbapenemase activity has been increasingly reported worldwide in A. baumannii. The aim of this study was to analyze the...

Université de Fribourg

Development of a kinetic assay for late endosome movement

Esner, Milan ; Meyenhofer, Felix ; Kuhn, Michael ; Thomas, Melissa ; Kalaidzidis, Yannis ; Bickle, Marc

In: Journal of Biomolecular Screening, 2014, vol. 19, no. 7, p. 1070–1078

Automated imaging screens are performed mostly on fixed and stained samples to simplify the workflow and increase throughput. Some processes, such as the movement of cells and organelles or measuring membrane integrity and potential, can be measured only in living cells. Developing such assays to screen large compound or RNAi collections is challenging in many respects. Here, we develop a...

Université de Fribourg

The difficult-to-control spread of carbapenemase producers in Enterobacteriaceae worldwide

Nordmann, Patrice ; Poirel, Laurent

In: Clinical Microbiology and Infection, 2014, p. -

Spread of carbapenemase producers in Enterobacteriaceae is now identified worldwide. Three main carbapenemases are reported which belong to three classes of ß-lactamases that are KPC, NDM and OXA-48. The main reservoirs of KPC are Klebsiella pneumoniae in the USA, Israel, Greece and Italy, of NDM are K. pneumoniae and Escherichia coli in the Indian subcontinent, and of OXA-48 are K. pneumoniae...

Université de Fribourg

Characteristics of Escherichia coli sequence type 131 isolates that produce extended-spectrum β-lactamases: global distribution of the H30-Rx sublineage

Peirano, Gisele ; Bij, Akke K. van der ; Freeman, Joshua L. ; Poirel, Laurent ; Nordmann, Patrice ; Costello, Michael ; Tchesnokova, Veronika L. ; Pitout, Johann D. D.

In: Antimicrobial Agents and Chemotherapy, 2014, vol. 58, no. 7, p. 3762–3767

We designed a study to describe the characteristics of sequence type 131 (ST131) lineages, including the H30-Rx sublineage, among a global collection of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from 9 countries collected from 2000 to 2011. A total of 240 nonrepeat isolates from Canada, the United States, Brazil, the Netherlands, France, the United Arab...