In: BMC Infectious Diseases, 2016, vol. 16, p. 275
Background: We investigated the molecular mechanism of ß-lactam resistance in extended-spectrum ß-lactamase (ESBL)-producing Enterobacterial strains isolated in neonatal units of different hospitals in Anatnanarivo, Madagascar.Methods: Bacteria were identified by standard biochemical methods, disc diffusion antibiograms and Etest. Resistance genes were sought by PCR. Strains were...
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In: Journal of Clinical Microbiology, 2016, p. JCM.00918-16
Enterobacterial strains resistant to polymyxins are increasingly reported worldwide. Conventional methods for detection of colistin-resistant isolates such as broth microdilution remain time-consuming (24 to 48 h), and methods such as disc diffusion and E-test are not reliable. Recently, the Rapid Polymyxin NP test was developed for a rapid identification of polymyxin-resistant...
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In: Antimicrobial Agents and Chemotherapy, 2016, vol. 60, no. 7, p. 4394–4397
A series of colistin-resistant Escherichia coli clinical isolates was recovered from hospitalized and community patients in South Africa. Seven clonally unrelated isolates harbored the mcr-1 gene located on different plasmid backbones. Two distinct plasmids were fully sequenced, and identical 2,600-bp-long DNA sequences defining a mcr-1 cassette were identified. Promoter sequences responsible...
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In: Clinical Microbiology and Infection, 2016, vol. 22, no. 5, p. 398–400
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In: Archives Suisses de Médecine Vétérinaire, 2016, vol. 158, no. 6, p. 457-460
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In: Journal of Antimicrobial Chemotherapy, 2016, p. dkw204
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In: The Lancet Infectious Diseases, 2016, vol. 16, no. 3, p. 281
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In: Journal of Antimicrobial Chemotherapy, 2016, p. dkw139
The aim of the study was to develop a simple assay for rapid detection of the mcr-1 gene, recently identified as a source of plasmid-mediated acquired resistance to polymyxins in Enterobacteriaceae.Methods: A SYBR Green-based real-time PCR assay was designed for detection of the mcr-1 gene. This assay was applied to cultured bacteria and to spiked human and cattle stools.Results: The mcr-1...
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In: Emerging Infectious Diseases, 2016, vol. 22, no. 6, p. -
For identification of polymyxin resistance in Enterobacteriaceae, we developed a rapid test that detects glucose metabolization associated with bacterial growth in the presence of a defined concentration of colistin or polymyxin B. Formation of acid metabolites is evidenced by a color change (orange to yellow) of a pH indicator (red phenol). To evaluate the test, we used bacterial colonies of...
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In: Antimicrobial Agents and Chemotherapy, 2016, vol. 60, no. 4, p. 2585–2586
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