Consortium of Swiss Academic Libraries

Transgenic mice and their impact on kidney research

Rubera, Isabelle ; Hummler, Edith ; Beermann, Friedrich

In: Pflügers Archiv - European Journal of Physiology, 2009, vol. 458, no. 1, p. 211-222

Université de Fribourg

Epithelial sodium channel-mediated sodium transport is not dependent on the membrane-bound serine protease CAP2/Tmprss4

Keppner, Anna ; Andreasen, Ditte ; Mérillat, Anne-Marie ; Bapst, Julie ; Ansermet, Camille ; Wang, Qing ; Maillard, Marc ; Malsure, Sumedha ; Nobile, Antoine ; Hummler, Edith

In: PLoS ONE, 2015, vol. 10, no. 8, p. e0135224

The membrane-bound serine protease CAP2/Tmprss4 has been previously identified in vitro as a positive regulator of the epithelial sodium channel (ENaC). To study its in vivo implication in ENaC-mediated sodium absorption, we generated a knockout mouse model for CAP2/Tmprss4. Mice deficient in CAP2/Tmprss4 were viable, fertile, and did not show any obvious histological abnormalities. Unexpectedly,...

Université de Fribourg

αENaC mediated lithium absorption promotes nephrogenic diabetes insipidus

Mønster Christensen, Birgitte ; Mercier Zuber, Annie ; Loffing, Johannes ; Stehle, Jean-Christophe ; Deen, Peter M.T. ; Rossier, Bernard C. ; Hummler, Edith

In: Journal of the American Society of Nephrology, 2010, vol. 21, p. -

Lithium-induced nephrogenic diabetes insipidus (NDI) is accompanied by polyuria, downregulation of aquaporin 2 (AQP2), and cellular remodeling of the collecting duct (CD). The amiloride-sensitive epithelial sodium channel (ENaC) is a likely candidate for lithium entry. Here, we subjected transgenic mice lacking αENaC specifically in the CD (knockout [KO] mice) and littermate controls to chronic...