Université de Fribourg

Respiratory hazard assessment of combined exposure to complete gasoline exhaust and respirable volcanic ash in a multicellular human lung model at the air-liquid interface

Tomašeka, Ines ; J.Horwell, Claire ; Bisig, Christoph ; E.Damby, David ; Comte, Pierre ; Czerwinski, Jan ; Petri-Finkb, Alke ; J.D.Clift, Martin ; Drasler, Barbara ; Rothen-Rutishauser, Barbara

In: Environmental Pollution, 2018, vol. 238, p. 977–987

Communities resident in urban areas located near active volcanoes can experience volcanic ash exposures during, and following, an eruption, in addition to sustained exposures to high concentrations of anthropogenic air pollutants (e.g., vehicle exhaust emissions). Inhalation of anthropogenic pollution is known to cause the onset of, or exacerbate, respiratory and cardiovascular diseases. It...

Université de Fribourg

Assessment of lung cell toxicity of various gasoline engine exhausts using a versatile in vitro exposure system

Bisig, Christoph ; Comte, Pierre ; Güdel, Martin ; Czerwinski, Jan ; Mayer, Andreas ; Müller, Loretta ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Environmental Pollution, 2018, vol. 235, p. 263–271

Adverse effect studies of gasoline exhaust are scarce, even though gasoline direct injection (GDI) vehicles can emit a high number of particles. The aim of this study was to conduct an in vitro hazard assessment of different GDI exhausts using two different cell culture models mimicking the human airway. In addition to gasoline particle filters (GPF), the effects of two lubrication oils with...

Université de Fribourg

Biological effects in lung cells In vitro of exhaust aerosols from a gasoline passenger car with and without particle filter

Bisig, Christoph ; Steiner, Sandro ; Comte, Pierre ; Czerwinski, Jan ; Mayer, Andreas ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Emission Control Science and Technology, 2015, vol. 1, no. 3, p. 237–246

Exhaust aerosol from gasoline passenger cars is a complex mixture of a particulate fraction as well as volatile compounds. In contrary to the observed adverse effects of diesel exhaust particles the gasoline exhaust has, however, received little attention so far. The aim of this study was to perform a comparison of exhaust composition and biological responses from freshly produced...

Université de Fribourg

Effects of an iron-based fuel-borne catalyst and a diesel particle filter on exhaust toxicity in lung cells in vitro

Steiner, Sandro ; Czerwinski, Jan ; Comte, Pierre ; Heeb, Norbert V. ; Mayer, Andreas ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Analytical and Bioanalytical Chemistry, 2015, vol. 407, no. 20, p. 5977–5986

Metal-containing fuel additives catalyzing soot combustion in diesel particle filters are used in a widespread manner, and with the growing popularity of diesel vehicles, their application is expected to increase in the near future. Detailed investigation into how such additives affect exhaust toxicity is therefore necessary and has to be performed before epidemiological evidence points towards...

Université de Fribourg

Test-methods on the test-bench: a comparison of complete exhaust and exhaust particle extracts for genotoxicity/mutagenicity assessment

Steiner, Sandro ; Heeb, Norbert V. ; Czerwinski, Jan ; Comte, Pierre ; Mayer, Andreas ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Environmental Science & Technology, 2014, vol. 48, no. 9, p. 5237–5244

With the growing number of new exhaust after-treatment systems, fuels and fuel additives for internal combustion engines, efficient and reliable methods for detecting exhaust genotoxicity and mutagenicity are needed to avoid the widespread application of technologies with undesirable effects toward public health. In a commonly used approach, organic extracts of particulates rather than complete...

Université de Fribourg

Reduction in (pro-)inflammatory responses of lung cells exposed in vitro to diesel exhaust treated with a non-catalyzed diesel particle filter

Steiner, Sandro ; Czerwinski, Jan ; Comte, Pierre ; Müller, Loretta L. ; Heeb, Norbert V. ; Mayer, Andreas ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Atmospheric Environment, 2013, vol. 81, p. 117–124

Increasingly stringent regulation of particulate matter emissions from diesel vehicles has led to the widespread use of diesel particle filters (DPFs), the effect of which on exhaust toxicity is so far poorly understood. We exposed a cellular model of the human respiratory epithelium at the air-liquid interface to non-catalyzed wall-flow DPF-filtered diesel exhaust and compared the resulting...

Université de Fribourg

Comparison of the toxicity of diesel exhaust produced by bio- and fossil diesel combustion in human lung cells in vitro

Steiner, Sandro ; Czerwinski, Jan ; Comte, Pierre ; Popovicheva, Olga ; Kireeva, Elena ; Müller, Loretta ; Heeb, Norbert ; Mayer, Andreas ; Fink, Alke ; Rothen-Rutishauser, Barbara

In: Atmospheric Environment, 2013, vol. 81, p. 380–388

Alternative fuels are increasingly combusted in diesel- and gasoline engines and the contribution of such exhausts to the overall air pollution is on the rise. Recent findings on the possible adverse effects of biodiesel exhaust are contradictive, at least partly resulting from the various fuel qualities, engine types and different operation conditions that were tested. However, most of the...