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Université de Fribourg

In vitro and in vivo characterization of noso-502, a novel inhibitor of bacterial translation

Racine, Emilie ; Nordmann, Patrice ; Pantel, Lucile ; Sarciaux, Matthieu ; Serri, Marine ; Houard, Jessica ; Villain-Guillot, Philippe ; Demords, Anthony ; Lundberg, Carina Vingsbo ; Gualtieri, Maxime

In: Antimicrobial Agents and Chemotherapy, 2018, vol. 62, no. 9, p. e01016-18

Antibacterial activity screening of a collection of Xenorhabdus strains led to the discovery of the odilorhabdins, a new antibiotic class with broad-spectrum activity against Gram-positive and Gram-negative pathogens. Odilorhabdins inhibit bacterial translation by a new mechanism of action on ribosomes. A lead optimization program identified NOSO-502 as a promising candidate. NOSO-502 has MIC...

Université de Fribourg

Screening and characterization of multidrug-resistant gram-negative bacteria from a remote african area, São Tomé and Príncipe

Poirel, Laurent ; Aires-de-Sousa, Marta ; Kudyba, Patrick ; Kieffer, Nicolas ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2018, vol. 62, no. 9, p. e01021-18

The occurrence of resistance to last-resort antibiotics was evaluated among Enterobacteriaceae isolates recovered from hospitalized children in a remote African archipelago, São Tomé and Príncipe, where there is limited access to those antibiotics. Fifty patients were screened for colonization by carbapenem-, pan- aminoglycoside-, or polymyxin-resistant Enterobacteriaceae. A total of 36...

Université de Fribourg

Evaluation of the Rapid Polymyxin NP test and its industrial version for the detection of polymyxin-resistant Enterobacteriaceae

Jayol, Aurélie ; Kieffer, Nicolas ; Poirel, Laurent ; Guérind, François ; Güneser, Deniz ; Cattoir, Vincent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 92, no. 2, p. 90–94

The commercial Rapid Polymyxin NP test was evaluated to detect colistin-resistant Enterobacteriaceae. A total of 223 enterobacterial isolates corresponding to 136 resistant (including 38 MCR-like producers), 19 heteroresistant, and 78 colistin- susceptible isolates were tested. The test was performed according to the manufacturer's instruction, and the color of the wells was read after 2 and 3...

Université de Fribourg

Detection of colistin-resistant Gram-negative rods by using the SuperPolymyxin medium

Jayol, Aurélie ; Poirel, Laurent ; André, Catherine ; Dubois, Véronique ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 92, no. 2, p. 95–101

The commercial SuperPolymyxin medium (ELITechGroup, Puteaux, France), currently licensed in Europe, is aimed to screen for either intrinsic or acquired colistin resistance in Gram negatives. It was tested for screening colistin-resistant Gram- negative rods from bacterial cultures of 145 colistin-resistant and 86 colistin- susceptible Gram-negative rods isolates. It exhibited a sensitivity and...

Université de Fribourg

Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil

Kieffer, Nicolas ; Nordmann, Patrice ; Moreno, Andrea Micke ; Zanolli Moreno, Luisa ; Chaby, Richard ; Breton, Aude ; Tissières, Pierre ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2018, vol. 62, no. 7, p. e00278-18

A collection of 126 pigs was screened for carriage of colistin-resistant Enterobacteriaceae in a farm in Minas Gerais, Brazil. Out of this collection, eight colistin-resistant Escherichia coli isolates were recovered, including one from Minas Gerais State producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a function similar to that of MCR-1...

Université de Fribourg

A culture medium for screening 16S rRNA methylase-producing pan-aminoglycoside resistant Gram-negative bacteria

Nordmann, Patrice ; Mazé, Alain ; Culebras, Esther ; Dobias, Jan ; Jayol, Aurélie ; Poirel, Laurent

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 91, no. 2, p. 118–122

The amikacin plus gentamicin-containing SuperAminoglycoside medium was developed for screening multiple-aminoglycoside resistance in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii). It was evaluated using aminoglycoside-susceptible (n=12) and aminoglycoside-resistant (n=59) Gram-negative isolates, including 16S rRNA methylase producers (n=20)....

Université de Fribourg

Integrase-mediated recombination of the bel-1 gene cassette encoding the extended-spectrum β-lactamase BEL-1

Bouheraoua, Nacim ; Poirel, Laurent ; Bourreau, Baptiste ; Bonnin, Rémy ; Laroche, Laurent ; Naas, Thierry ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2018, p. AAC.00030-18

Integrons are genetic elements that can acquire and rearrange gene cassettes. The blaBEL-1 gene encodes an extended-spectrum β-lactamase, BEL-1, that is present at second position of the variable region of class 1 integrons identified in Pseudomonas aeruginosa. The mobility of the bel-1 gene cassette was analyzed in physiological conditions and with the integrase gene being overexpressed....

Université de Fribourg

High rate of association of 16S rRNA methylases and carbapenemases in enterobacteriaceae recovered from hospitalized children in angola

Poirel, Laurent ; Goutines, Juliette ; Aires-de-Sousa, Marta ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2018, vol. 62, no. 4, p. e00021-18

Acquired 16S rRNA methylases (RMTases) conferring pan-drug resistance to aminoglycosides were searched among enterobacterial isolates recovered in Angola. A total of 36 hospitalized children were screened for rectal colonization using the Superaminoglycoside selective medium. Twenty-two pan-aminoglycoside-resistant enterobacterial isolates were recovered, all of which produced RMTases, i.e.,...

Université de Fribourg

Evaluation of three broth microdilution systems to determine colistin susceptibility of Gram-negative bacilli

Jayol, Aurélie ; Nordmann, Patrice ; André, Catherine ; Poirel, Laurent ; Dubois, Véronique

In: Journal of Antimicrobial Chemotherapy, 2018, p. -

The broth microdilution (BMD) method is currently the recommended technique to determine susceptibility to colistin.Objectives: We evaluated the accuracy of three commercialized BMD panels [Sensititre (ThermoFisher Diagnostics), UMIC (Biocentric) and MicroScan (Beckman Coulter)] to determine colistin susceptibility.Methods: A collection of 185 isolates of Gram-negative bacilli (133 colistin...