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Université de Fribourg

A resazurin reduction-based assay for rapid detection of polymyxin resistance in Acinetobacter baumannii and Pseudomonas aeruginosa

Lescat, Mathilde ; Poirel, Laurent ; Tinguely, Camille ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2018, p. -

A rapid test was developed for identification of polymyxin resistance in non-fermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin. The principle of this test is based on the visual detection of the reduction of the resazurin reagent, a viability colorant, observed by its color change (blue to purple or pink). Its...

Université de Fribourg

Screening for Intestinal Carriage of Extended-spectrum Beta-lactamase–producing Enterobacteriaceae in Critically Ill Patients: Expected Benefits and Evidence-based Controversies

Zahar, Jean-Ralph ; Blot, Stijn ; Nordmann, Patrice ; Martischang, Romain ; Timsit, Jean-François ; Harbarth, Stephan ; Barbier, François

In: Clinical Infectious Diseases, 2018, p. -

The rising burden of intensive care unit (ICU)-acquired infections due to extended- spectrum beta-lactamase–producing Enterobacteriaceae (ESBL-E) strengthens the requirement for efficient prevention strategies. The detection of intestinal carriage of ESBL-E through active surveillance cultures (ASC) and the implementation of contact precautions (CP) in carriers are currently advocated in...

Université de Fribourg

Rapid detection of fosfomycin resistance in Escherichia coli

Nordmann, Patrice ; Poirel, Laurent ; Mueller, Linda

In: Journal of Clinical Microbiology, 2019, vol. 57, no. 1, p. e01531-18

The rapid fosfomycin/Escherichia coli NP test was developed to detect fosfomycin resistance in E. coli isolates. The test is based on glucose metabolization and the detection of bacterial growth in the presence of fosfomycin at 40 µg/ml. Bacterial growth is visually detectable by an orange-to-yellow color change of red phenol, a pH indicator. A total of 100 E. coli isolates, among which 22...

Université de Fribourg

Antimicrobial Resistance in Escherichia coli

Poirel, Laurent ; Madec, Jean-Yves ; Lupo, Agnese ; Schink, Anne-Kathrin ; Kieffer, Nicolas ; Nordmann, Patrice ; Schwarz, Stefan

In: Microbiology Spectrum, 2018, vol. 6, no. 4, p. -

Multidrug resistance in Escherichia coli has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. E. coli is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic...

Université de Fribourg

Acquisition of extended-spectrum β-lactamase GES-6 leading to resistance to ceftolozane-tazobactam combination in Pseudomonas aeruginosa

Poirel, Laurent ; Ortiz De La Rosa, José-Manuel ; Kieffer, Nicolas ; Dubois, Véronique ; Jayol, Aurélie ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2019, vol. 63, no. 1, p. e01809-18

A clinical Pseudomonas aeruginosa isolate resistant to all β-lactams, including ceftolozane-tazobactam and carbapenems, was recovered. It belonged to sequence type 235 and produced the extended-spectrum β-lactamase (ESBL) GES-6 differing from GES-1 by two amino acid substitutions (E104K and G170S). GES-6 possessed an increased hydrolytic activity toward carbapenems and to ceftolozane and a ...

Université de Fribourg

Stability of cefiderocol against clinically significant broad-spectrum oxacillinases

Poirel, Laurent ; Kieffer, Nicolas ; Nordmann, Patrice

In: International Journal of Antimicrobial Agents, 2018, vol. 52, no. 6, p. 866–867

Université de Fribourg

Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes

Lescata, Mathilde ; Poirel, Laurent ; Nordmann, Patrice

In: Diagnostic Microbiology and Infectious Disease, 2018, vol. 92, no. 4, p. 267–269

Université de Fribourg

Performances of the rapid polymyxin Acinetobacter and Pseudomonas tests for colistin susceptibility testing

Lescat, Mathilde ; Poirel, Laurent ; Jayol, Aurélie ; Nordmann, Patrice

In: Microbial Drug Resistance, 2018, p. -

Owing to the emergence of colistin resistance in nonfermenting Gram negative bacteria, reliable and rapid techniques for testing colistin susceptibility are needed. We evaluated the performances of the Rapid Polymyxin Acinetobacter and Pseudomonas tests using a collection of Acinetobacter baumannii and Pseudomonas aeruginosa clinical isolates.Methods: Colistin susceptibility of A. baumannii...

Université de Fribourg

In vitro and in vivo characterization of noso-502, a novel inhibitor of bacterial translation

Racine, Emilie ; Nordmann, Patrice ; Pantel, Lucile ; Sarciaux, Matthieu ; Serri, Marine ; Houard, Jessica ; Villain-Guillot, Philippe ; Demords, Anthony ; Lundberg, Carina Vingsbo ; Gualtieri, Maxime

In: Antimicrobial Agents and Chemotherapy, 2018, vol. 62, no. 9, p. e01016-18

Antibacterial activity screening of a collection of Xenorhabdus strains led to the discovery of the odilorhabdins, a new antibiotic class with broad-spectrum activity against Gram-positive and Gram-negative pathogens. Odilorhabdins inhibit bacterial translation by a new mechanism of action on ribosomes. A lead optimization program identified NOSO-502 as a promising candidate. NOSO-502 has MIC...