Université de Fribourg

Estimating and accounting for genotyping errors in RAD-seq experiments

Bresadola, Luisa ; Link, Vivian ; Buerkle, C. Alex ; Lexer, Christian ; Wegmann, Daniel

In: Molecular Ecology Resources, 2020, vol. 20, no. 4, p. 856–870

In non-model organisms, evolutionary questions are frequently addressed using reduced representation sequencing techniques due to their low cost, ease of use, and because they do not require genomic resources such as a reference genome. However, evidence is accumulating that such techniques may be affected by specific biases, questioning the accuracy of obtained genotypes, and as a...

Université de Fribourg

Admixture mapping in interspecific Populus hybrids identifies classes of genomic architectures for phytochemical, morphological and growth traits

Bresadola, Luisa ; Caseys, Céline ; Castiglione, Stefano ; Buerkle, C. Alex ; Wegmann, Daniel ; Lexer, Christian

In: New Phytologist, 2019, vol. 223, no. 4, p. 2076–2089

The genomic architecture of functionally important traits is key to understanding the maintenance of reproductive barriers and trait differences when divergent populations or species hybridize. We conducted a genome-wide association study (GWAS) to study trait architecture in natural hybrids of two ecologically divergent Populus species. We genotyped 472 seedlings from a natural hybrid zone...

Université de Fribourg

Unexpected ancestry of Populus seedlings from a hybrid zone implies a large role for postzygotic selection in the maintenance of species

Lindtke, Dorothea ; Gompert, Zachariah ; Lexer, Christian ; Buerkle, C. Alex

In: Molecular Ecology, 2014, p. –

In the context of potential interspecific gene flow, the integrity of species will be maintained by reproductive barriers that reduce genetic exchange, including traits associated with prezygotic isolation or poor performance of hybrids. Hybrid zones can be used to study the importance of different reproductive barriers, particularly when both parental species and hybrids occur in close spatial...

Université de Fribourg

Recombinant hybrids retain heterozygosity at many loci: new insights into the genomics of reproductive isolation in Populus

Lindtke, Dorothea ; Buerkle, C. Alex ; Barbará, Thelma ; Heinze, Berthold ; Castiglione, Stefano ; Bartha, Denes ; Lexer, Christian

In: Molecular Ecology, 2012, p. -

The maintenance of species barriers in the face of gene flow is often thought to result from strong selection against intermediate genotypes, thereby preserving genetic differentiation. Most speciation genomic studies thus aim to identify exceptionally divergent loci between populations, but divergence will be affected by many processes other than reproductive isolation (RI) and speciation....

Université de Fribourg

Genomic admixture analysis in european Populus spp. reveals unexpected patterns of reproductive isolation and mating

Lexer, Christian ; Joseph, Jeffrey A. ; Loo, Marcela van ; Barbará, Thelma ; Heinze, Berthold ; Bartha, Denes ; Castiglione, Stefano ; Fay, Michael F. ; Buerkle, C. Alex

In: Genetics, 2010, vol. 186, p. 699–712

Admixture between genetically divergent populations facilitates genomic studies of the mechanisms involved in adaptation, reproductive isolation and speciation, including mapping of the loci involved in these phenomena. Little is known about how pre- and postzygotic barriers will affect the prospects of 'admixture mapping' in wild species. We have studied 93 mapped genetic markers...

Université de Fribourg

Admixture as the basis for genetic mapping

Buerkle, C. Alex ; Lexer, Christian

In: Trends in Ecology & Evolution, 2008, vol. 23, no. 12, p. 686-694

Genetic mapping in natural populations is increasing rapidly in feasibility and accessibility. As with many areas in genetics, advances in molecular techniques and statistics are drastically altering how we can investigate inheritance in wild organisms. For ecology and evolution, this is particularly significant and promising, because many of the organisms of interest are not amenable to...