In: Journal of Neurophysiology, 2011, vol. 106, no. 4, p. 1793-1805
We examined the relationship between somatic Ca²⁺ signals and spiking activity of cerebellar molecular layer interneurons (MLIs) in adult mice. Using two-photon microscopy in conjunction with cell-attached recordings in slices, we show that in tonically firing MLIs loaded with high-affinity Ca²⁺ probes, Ca²⁺-dependent fluorescence transients are absent. Spike-triggered averages of...
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In: The Anatomical Record, 2008, vol. 291, no. 5, p. 538 - 546
The well-organized cerebellum is an ideal model to investigate the developmental appearance and localization of pre- and postsynaptic structures. One of the synaptic proteins abundant in the central nervous system and localized in presynaptic vesicle membranes is the synaptic vesicle protein 2 (SV2). SV2 was shown to be involved in priming and modulating synaptic vesicles and having an effect in...
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In: Molecular and Cellular Neuroscience, 2008/38/4/10.1016/j.mcn.2008.04.010
Engrailed transcription factors regulate survival, cell fate decisions and axon pathfinding in central neurons. En-2 can also attenuate Purkinje cell (PC) maturation. Here, we use array analysis to scrutinize gene expression in developing PCs overexpressing Engrailed-2 (L7En-2). The majority (70%) of regulated genes was found down-regulated in L7En-2 cerebella, consistent with the known...
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In: The Journal of Neuroscience, 2005, vol. 25, no. 1, p. 96-107
Certain interneurons contain large concentrations of specific Ca²⁺-binding proteins (CBPs), but consequences on presynaptic Ca²⁺ signaling are poorly understood. Here we show that expression of the slow CBP parvalbumin (PV) in cerebellar interneurons is cell specific and developmentally regulated, leading to characteristic changes in presynaptic Ca²⁺ dynamics (Cai). Using...
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