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Université de Fribourg

PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae

Baccarini, Leticia ; Martínez-Montañés, Fernando ; Rossi, Silvia ; Proft, Markus ; Portela, Paula

In: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2015, vol. 1849, no. 11, p. 1329–1339

Gene expression regulation by intracellular stimulus-activated protein kinases is essential for cell adaptation to environmental changes. There are three PKA catalytic subunits in Saccharomyces cerevisiae: Tpk1, Tpk2, and Tpk3 and one regulatory subunit: Bcy1. Previously, it has been demonstrated that Tpk1 and Tpk2 are associated with coding regions and promoters of target genes in a carbon...

Université de Fribourg

Multiple functions of inositolphosphorylceramides in the formation and intracellular transport of glycosylphosphatidylinositol-anchored proteins in yeast

Bosson, Régine ; Conzelmann, Andreas

In: Biochemical Society Symposia, 2007, vol. 74, p. 199–209

The mature sphingolipids of yeast consist of IPCs (inositolphosphorylceramides) and glycosylated derivatives thereof. Beyond being an abundant membrane constituent in the organelles of the secretory pathway, IPCs are also used to constitute the lipid moiety of the majority of GPI (glycosylphosphatidylinositol) proteins, while a minority of GPI proteins contain PI (phosphatidylinositol). Thus all...

Université de Fribourg

The caveolin-binding motif of the pathogen-related yeast protein Pry1, a member of the CAP protein superfamily, is required for in vivo export of cholesteryl acetate

Choudhary, Vineet ; Darwiche, Rabih ; Gfeller, David ; Zoete, Vincent ; Michielin, Olivier ; Schneiter, Roger

In: Journal of Lipid Research, 2014, vol. 55, no. 5, p. 883–894

Proteins belonging to the CAP superfamily are present in all kingdoms of life and have been implicated in different physiological processes. Their molecular mode of action, however, is poorly understood. Saccharomyces cerevisiae expresses three members of this superfamily, pathogen-related yeast (Pry)1, -2, and -3. We have recently shown that Pry function is required for the secretion of...

Université de Fribourg

Monitoring sterol uptake, acetylation, and export in yeast

Choudhary, Vineet ; Schneiter, Roger

In: Methods in Molecular Biology, 2009, vol. 580, p. 221-232

Sterols are essential lipid components of eukaryotic membranes. They are synthesized in the endoplasmatic reticulum (ER) from where they are efficiently transported to the plasma membrane, which harbors ~90% of the free sterol pool of the cell. The molecular mechanisms that govern this lipid transport, however, are not well characterized and are challenging to analyze. Saccharomyces cerevisiae...

Université de Fribourg

Lipid droplets are functionally connected to the endoplasmic reticulum in Saccharomyces cerevisiae

Jacquier, Nicolas ; Choudhary, Vineet ; Mari, Muriel ; Toulmay, Alexandre ; Health and Human Services, Bethesda, USA ; Reggiori, Fulvio ; Schneiter, Roger

In: Journal of Cell Science, 2011, vol. 124, p. 2424-2437

Cells store metabolic energy in the form of neutral lipids that are deposited within lipid droplets (LDs). In this study, we examine the biogenesis of LDs and the transport of integral membrane proteins from the endoplasmic reticulum (ER) to newly formed LDs. In cells that lack LDs, otherwise LD-localized membrane proteins are homogenously distributed in the ER membrane. Under these conditions,...

Université de Fribourg

Schistosoma mansoni venom allergen-like protein 4 (SmVAL4) is a novel lipid-binding SCP/TAPS protein that lacks the prototypical CAP motifs

Kelleher, Allan ; Darwiche, Rabih ; Rezende, Wanderson C. ; Farias, Leonardo P. ; Leite, Luciana C. C. ; Schneiter, Roger ; Asojo, Oluwatoyin A.

In: Acta Crystallographica Section D Biological Crystallography, 2014, vol. 70, no. 8, p. 2186–2196

Schistosomiasis is a parasitic disease that affects over 200 million people. Vaccine candidates have been identified, including Schistosoma mansoni venom allergen- like proteins (SmVALs) from the SCP/TAPS (sperm-coating protein/Tpx/antigen 5/pathogenesis related-1/Sc7) superfamily. The first SmVAL structure, SmVAL4, was refined to a resolution limit of 2.16 Å. SmVAL4 has a unique...

Université de Fribourg

Biosynthesis and function of GPI proteins in the yeast Saccharomyces cerevisiae

Pittet, Martine ; Conzelmann, Andreas

In: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2007, vol. 1771, no. 3, p. 405-420

Like most other eukaryotes, Saccharomyces cerevisiae harbors a GPI anchoring machinery and uses it to attach proteins to membranes. While a few GPI proteins reside permanently at the plasma membrane, a majority of them gets further processed and is integrated into the cell wall by a covalent attachment to cell wall glucans. The GPI biosynthetic pathway is necessary for growth and survival...

Université de Fribourg

Saccharomyces cerevisiae, a model to study sterol uptake and transport in eukaryotes

Reiner, Sonja ; Micolod, Delphine ; Schneiter, Roger

In: Biochemical Society Transactions, 2005, vol. 33, p. 1186

The molecular mechanisms that govern intracellular transport of sterols in eukaryotic cells are only poorly understood. Saccharomyces cerevisiae is a facultative anaerobic organism that requires supplementation with unsaturated fatty acids and sterols to grow in the absence of oxygen, as the synthesis of these lipids requires molecular oxygen. The fact that yeast grows well under anaerobic...

Université de Fribourg

Transcriptome structure variability in Saccharomyces cerevisiae strains determined with a newly developed assembly software

Sardu, Alessandro ; Treu, Laura ; Campanaro, Stefano

In: BMC Genomics, 2014, vol. 15, no. 1, p. 1045

RNA-seq studies have an important role for both large-scale analysis of gene expression and for transcriptome reconstruction. However, the lack of software specifically developed for the analysis of the transcriptome structure in lower eukaryotes, has so far limited the comparative studies among different species and strains.Results: In order to fill this gap, an innovative software called ORA...