Refine my results

Document type

Specific Collection

Language

Université de Fribourg

Complete genome sequence of the clinical strain Acinetobacter baumannii R2090 Carrying the chromosomally encoded Metallo-β-Lactamase Gene blaNDM-1

Krahn, Thomas ; Wibberg, Daniel ; Maus, Irena ; Winkler, Anika ; Nordmann, Patrice ; Pühler, Alfred ; Poirel, Laurent ; Schlüter, Andreas

In: Genome Announcements, 2016, vol. 3, no. 5, p. e01008-15

Acinetobacter baumannii is an emerging human pathogen causing nosocomial and community-acquired infections. Here, we present the complete genome sequence of the clinical A. baumannii strain R2090 carrying the metallo-β-lactamase gene blaNDM-1 in its chromosome within the transposon Tn125.

Université de Fribourg

Acquisition of broad-spectrum cephalosporin resistance leading to colistin resistance in Klebsiella pneumoniae

Jayol, Aurélie ; Nordmann, Patrice ; Desroches, Marine ; Decousser, Jean-Winoc ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2016, vol. 60, no. 5, p. 3199–3201

An extended-spectrum β-lactamase (ESBL)-producing and colistin-resistant Klebsiella pneumoniae clinical isolate was recovered from a patient who was treated with cefotaxime. This isolate harbored a blaCTX-M-15 ESBL gene that was associated with an ISEcp1 insertion sequence. Transposition of that tandem occurred within the chromosomal mgrB gene, leading...

Université de Fribourg

A Universal culture medium for screening polymyxin-resistant gram-negative isolates

Nordmann, Patrice ; Jayol, Aurélie ; Poirel, Laurent

In: Journal of Clinical Microbiology, 2016, vol. 54, no. 5, p. 1395–1399

The colistin-containing SuperPolymyxin medium was developed for screening polymyxin-resistant Gram-negative bacteria. It was evaluated with 88 polymyxin- susceptible or polymyxin-resistant cultured Gram-negative isolates. Its sensitivity and specificity of detection were ca. 100%. The SuperPolymyxin medium is the first screening medium that is able to detect intrinsic and acquired...

Université de Fribourg

Chromobacterium spp. harbour Ambler class A β-lactamases showing high identity with KPC

Gudeta, Dereje Dadi ; Bortolaia, Valeria ; Jayol, Aurélie ; Poirel, Laurent ; Nordmann, Patrice ; Guardabassi, Luca

In: Journal of Antimicrobial Chemotherapy, 2016, p. -

Objectives The origin of KPC is unknown. The aim of this study was to detect progenitors of KPC in silico and to functionally verify their β-lactam hydrolysis activity.Methods The sequence of KPC-2 was used to mine the NCBI protein sequence database. The best non-KPC hits were analysed by amino acid (aa) alignment and phylogenetic tree construction....

Université de Fribourg

First detection of Klebsiella variicola producing OXA-181 carbapenemase in fresh vegetable imported from Asia to Switzerland

Zurfluh, K. ; Poirel, Laurent ; Nordmann, Patrice ; Klumpp, J. ; Stephan, R.

In: Antimicrobial Resistance and Infection Control, 2015, vol. 4, p. 38

Background: The emergence and worldwide spread of carbapenemase-producing Enterobacteriaceae is of great concern to public health services. The aim of this study was to investigate the occurrence of carbapenemase-producing Enterobacteriaceae in fresh vegetables and spices imported from Asia to Switzerland.Findings: Twenty-two different fresh vegetable samples were purchased in March 2015 from...

Université de Fribourg

In vitro evaluation of dual carbapenem combinations against carbapenemase-producing Enterobacteriaceae

Poirel, Laurent ; Kieffer, Nicolas ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2016, vol. 71, no. 1, p. 156–161

Objectives: This study aimed to analyse the in vitro activity of dual combinations of carbapenems against Klebsiella pneumoniae producing the main carbapenemase types.Methods: MIC values of the carbapenems, imipenem, meropenem, ertapenem and doripenem were determined alone and in dual combinations for 20 clinical K. pneumoniae isolates producing representative carbapenemases, i.e. OXA-48...

Université de Fribourg

Dissemination of multiresistant Enterobacter cloacae isolates producing OXA-48 and CTX-M-15 in a Spanish hospital

Fernández, Javier ; Montero, Ignacio ; Martínez, Óscar ; Fleites, Ana ; Poirel, Laurent ; Nordmann, Patrice ; Rodicio, M. Rosario

In: International Journal of Antimicrobial Agents, 2015, vol. 46, no. 4, p. 469–474

Twenty-one multiresistant Enterobacter cloacae isolates producing OXA-48 (n = 10), CTX-M-15 (n = 7) or both (n = 4) β-lactamases were detected in a Spanish hospital during a 1-year period (June 2013 to June 2014). The isolates were also resistant to non-β-lactam antimicrobials, further complicating the therapeutic options. Genotyping of the isolates...

Université de Fribourg

Comparison of three biochemical tests for rapid detection of extended-spectrum β-lactamase-producing Enterobacteriaceae

Poirel, Laurent ; Fernández, Javier ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2015, p. JCM.01840–15

Enterobacterial isolates producing clavulanic-inhibited extended-spectrum β-lactamases (ESBLs) are increasingly spreading in the community and are often responsible for nosocomial infections. Rapid biochemical tests have been developed recently for their detection. Three tests, namely the Rapid ESBL NDP test, the β-Lacta test and the Rapid ESBL Screen have been evaluated with a collection of...