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Università della Svizzera italiana

Enhancing multi-scale cardiac simulations by coupling electrophysiology and mechanics : a flexible high performance approach to cardiac electromechanics

Pozzi, Sonia ; Krause, Rolf (Dir.)

Thèse de doctorat : Università della Svizzera italiana, 2019 ; 2019INFO005.

This work focuses on the development of computational methods for the simulation of the propagation of the electrical potential in the heart and of the resulting mechanical contraction. The interaction of these two physical phenomena is described by an electromechanical model which consists of the monodomain system, which describes the propagation of the action potential in the cardiac tissue,...

Università della Svizzera italiana

Characterization of neutralizing profiles in HIV-1 infected patients from whom the HJ16, HGN194 and HK20 mAbs were obtained

Balla-Jhagjhoorsingh, Sunita S. ; Willems, Betty ; Heyndrickx, Liesbeth ; Heyndrickx, Leo ; Vereecken, Katleen ; Janssens, Wouter ; Seaman, Michael S. ; Corti, Davide ; Lanzavecchia, Antonio ; Davis, David ; Vanham, Guido

In: Plos one, 2011, vol. 6, no. 10, p. e25488

Several new human monoclonal antibodies (mAbs) with a neutralizing potential across different subtypes have recently been described. Three mAbs, HJ16, HGN194 and HK20, were obtained from patients within the HIV-1 cohort of the Institute of Tropical Medicine (ITM). Our aim was to generate immunization antibodies equivalent to those seen in plasma. Here, we describe the selection and...

Università della Svizzera italiana

Unconventional use of LC3 by coronaviruses through the alleged subversion of the ERAD tuning pathway

Reggiori, Fulvio ; De Haan, Cornelis A.M. ; Molinari, Maurizio

In: Viruses, 2011, vol. 3, no. 9, p. 1610-1623

Pathogens of bacterial and viral origin hijack pathways operating in eukaryotic cells in many ways in order to gain access into the host, to establish themselves and to eventually produce their progeny. The detailed molecular characterization of the subversion mechanisms devised by pathogens to infect host cells is crucial to generate targets for therapeutic intervention. Here we review recent...

Università della Svizzera italiana

HTLV-1 propels thymic human T cell development in “human immune system” Rag2-/- gamma c-/- Mice

Villaudy, Julien ; Wencker, Mélanie ; Gadot, Nicolas ; Gillet, Nicolas A. ; Scoazec, Jean-Yves ; Gazzolo, Louis ; Manz, Markus G. ; Bangham, Charles R. M. ; Duc Dodon, Madeleine

In: Plos pathogens, 2011, vol. 7, no. 9, p. e1002231

Alteration of early haematopoietic development is thought to be responsible for the onset of immature leukemias and lymphomas. We have previously demonstrated that TaxHTLV-1 interferes with ß- selection, an important checkpoint of early thymopoiesis, indicating that human T-cell leukemia virus type 1 (HTLV-1) infection has the potential to perturb thymic human αβ T-cell development. To...

Università della Svizzera italiana

T regulatory cells are markers of disease activity in multiple sclerosis patients

Dalla Libera, Dacia ; Di Mitri, Diletta ; Bergami, Alessandra ; Centonze, Diego ; Gasperini, Claudio ; Grasso, Maria Grazia ; Galgani, Simona ; Martinelli, Vittorio ; Comi, Giancarlo ; Avolio, Carlo ; Martino, Gianvito ; Borsellino, Giovanna ; Sallusto, Federica ; Battistini,Luca ; Furlan, Roberto

In: Plos one, 2011, vol. 6, no. 6, p. e21386

FoxP3+ Treg cells are believed to play a role in the occurrence of autoimmunity and in the determination of clinical recurrences. Contradictory reports are, however, available describing frequency and function of Treg cells during autoimmune diseases. We examined, by both polychromatic flow cytometry, and real-time RT-PCR, several Treg markers in peripheral blood mononuclear cells from...

Università della Svizzera italiana

In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection

Alwis, Ruklanthi de ; Beltramello, Martina ; Messer, William B. ; Sukupolvi-Petty, Soila ; Wahala, Wahala M. P. B. ; Kraus, Annette ; Olivarez, Nicholas P. ; Pham, Quang ; Brian, James ; Tsai, Wen-Yang ; Wang, Wei-Kung ; Halstead, Scott ; Kliks, Srisakul ; Diamond, Michael S. ; Baric, Ralph ; Lanzavecchia, Antonio ; Sallusto, Federica ; Silva, Aravinda M. de

In: Plos neglected tropical diseases, 2011, vol. 5, no. 6, p. e1188

Humans who experience a primary dengue virus (DENV) infection develop antibodies that preferentially neutralize the homologous serotype responsible for infection. Affected individuals also generate cross-reactive antibodies against heterologous DENV serotypes, which are non-neutralizing. Dengue cross-reactive, non-neutralizing antibodies can enhance infection of Fc receptor bearing cells and,...

Università della Svizzera italiana

Chronic delivery of antibody fragments using immunoisolated cell implants as a passive vaccination tool

Belaunzaran, Osiris Marroquin ; Cordero, Maria Isabel ; Setola, Veronica ; Bianchi, Siro ; Galli, Carmela ; Bouche, Nicolas ; Mlynarik, Vladimir ; Gruetter, Rolf ; Sandi, Carmen ; Bensadoun, Jean-Charles ; Molinari, Maurizio ; Aebischer, Patrick

In: Plos one, 2011, vol. 6, no. 4, p. e18268

Background: Monoclonal antibodies and antibody fragments are powerful biotherapeutics for various debilitating diseases. However, high production costs, functional limitations such as inadequate pharmacokinetics and tissue accessibility are the current principal disadvantages for broadening their use in clinic. Methodology and Principal Findings: We report a novel method for the long-term ...

Università della Svizzera italiana

An anti-HIV-1 V3 loop antibody fully protects cross-clade and elicits T-cell immunity in macaques mucosally challenged with an R5 clade C SHIV

Watkins, Jennifer D. ; Siddappa, Nagadenahalli B. ; Lakhashe, Samir K. ; Humbert, Michael ; Sholukh, Anton ; Hemashettar, Girish ; Wong, Yin Ling ; Yoon, John K. ; Wang, Wendy ; Novembre, Francis J. ; Villinger, Francois ; Ibegbu, Chris ; Patel, Kalpana ; Corti, Davide ; Agatic, Gloria ; Vanzetta, Fabrizio ; Bianchi, Siro ; Heeney, Jonathan L. ; Sallusto, Federica ; Lanzavecchia, Antonio ; Ruprecht, Ruth M.

In: Plos one, 2011, vol. 6, no. 3, p. e18207

Neutralizing antibodies have been shown to protect macaques against SHIV challenge. However, genetically diverse HIV-1 clades have evolved, and a key question left unanswered is whether neutralizing antibodies can confer cross-clade protection in vivo. The novel human monoclonal antibody HGN194 was isolated from an individual infected with an HIV-1 clade AG recombinant circulating recombinant...

Università della Svizzera italiana

Improved innate and adaptive immunostimulation by genetically modified HIV-1 protein expressing NYVAC vectors

Quakkelaar, Esther D. ; Redeker, Anke ; Haddad, Elias K. ; Harari, Alexandre ; McCaughey, Stella Mayo ; Duhen, Thomas ; Filali-Mouhim, Abdelali ; Goulet, Jean-Philippe ; Loof, Nikki M. ; Ossendorp, Ferry ; Perdiguero, Beatriz ; Heinen, Paul ; Gomez, Carmen E. ; Kibler, Karen V. ; Koelle, David M. ; Sékaly, Rafick P. ; Sallusto, Federica ; Lanzavecchia, Antonio ; Pantaleo, Giuseppe ; Esteban, Mariano ; Tartaglia, Jim ; Jacobs, Bertram L. ; Melief, Cornelis J. M.

In: Plos one, 2011, vol. 6, no. 2, p. e16819

Attenuated poxviruses are safe and capable of expressing foreign antigens. Poxviruses are applied in veterinary vaccination and explored as candidate vaccines for humans. However, poxviruses express multiple genes encoding proteins that interfere with components of the innate and adaptive immune response. This manuscript describes two strategies aimed to improve the immunogenicity of the...

Università della Svizzera italiana

Computational docking of antibody-antigen complexes, opportunities and pitfalls illustrated by influenza hemagglutinin

Pedotti, Mattia ; Simonelli, Luca ; Livoti, Elsa ; Varani, Luca

In: International journal of molecular sciences, 2011, vol. 12, no. 1, p. 226-251

Antibodies play an increasingly important role in both basic research and the pharmaceutical industry. Since their efficiency depends, in ultimate analysis, on their atomic interactions with an antigen, studying such interactions is important to understand how they function and, in the long run, to design new molecules with desired properties. Computational docking, the process of predicting...