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Using Natural Variation to Investigate the Function of Individual Amino Acids in the Sucrose-Binding Box of Fructan:Fructan 6G-Fructosyltransferase (6G-FFT) in Product Formation

Ritsema, Tita ; Verhaar, Auke ; Vijn, Irma ; Smeekens, Sjef

In: Plant Molecular Biology, 2005, vol. 58, no. 5, p. 597-607

Engineering yeast alcohol dehydrogenase. Replacing Trp54 by Leu broadens substrate specificity

Weinhold, Elmar G. ; Benner, Steven A.

In: Protein Engineering, Design and Selection, 1995, vol. 8, no. 5, p. 457-461

A hybrid of bovine pancreatic ribonuclease and human angiogenin: an external loop as a module controlling substrate specificity?

Allemann, Rudolf K. ; Presnell, Scott R. ; Benner, Steven A.

In: Protein Engineering, Design and Selection, 1991, vol. 4, no. 7, p. 831-835

A two-step method for the introduction of single or multiple defined point mutations into the genome of Saccharomyces cerevisiae

Toulmay, Alexandre ; Schneiter, Roger

In: Yeast, 2006, vol. 23, no. 11, p. 825-831

The introduction of defined mutations into open reading frames (ORF) or non-translated regions of the genome is important to study of the structure-function relationship of amino acid residues in proteins or that of sequence motifs at the genome level. We describe a simple two-step method for the introduction of defined single or multiple point mutations into the genome of Saccharomyces...