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Università della Svizzera italiana

Fetal human cytomegalovirus transmission correlates with delayed maternal antibodies to gH/gL/pUL128-130-131 complex during primary infection

Lilleri, Daniele ; Kabanova, Anna ; Revello, Maria Grazia ; Percivalle, Elena ; Sarasini, Antonella ; Genini, Emilia ; Sallusto, Federica ; Lanzavecchia, Antonio ; Corti, Davide ; Gerna, Giuseppe

In: Plos one, 2013, vol. 8, no. 3, p. e59863

Primary human cytomegalovirus (HCMV) infections during pregnancy are associated with a high risk of virus transmission to the fetus. To identify correlates of intrauterine HCMV transmission, serial serum samples from HCMV transmitter and non-transmitter pregnant women with primary HCMV infection were analyzed for the presence of neutralizing antibodies against different glycoproteins and...

Università della Svizzera italiana

Therapeutic efficacy of antibodies lacking FcγR against lethal dengue virus infection is due to neutralizing potency and blocking of enhancing antibodies

Williams, Katherine L. ; Sukupolvi-Petty, Soila ; Beltramello, Martina ; Johnson, Syd ; Sallusto, Federica ; Lanzavecchia, Antonio ; Diamond, Michael S. ; Harris, Eva

In: Plos pathogens, 2013, vol. 9, no. 2, p. e1003157

Dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS) are life-threatening complications following infection with one of the four serotypes of dengue virus (DENV). At present, no vaccine or antiviral therapies are available against dengue. Here, we characterized a panel of eight human or mouse-human chimeric monoclonal antibodies (MAbs) and their modified variants lacking effector...

Università della Svizzera italiana

Rational engineering of a human anti-dengue antibody through experimentally validated computational docking

Simonelli, Luca ; Pedotti, Mattia ; Beltramello, Martina ; Livoti, Elsa ; Calzolai, Luigi ; Sallusto, Federica ; Lanzavecchia, Antonio ; Varani, Luca

In: Plos one, 2013, vol. 8, no. 2, p. e55561

Antibodies play an increasing pivotal role in both basic research and the biopharmaceutical sector, therefore technology for characterizing and improving their properties through rational engineering is desirable. This is a difficult task thought to require high-resolution x-ray structures, which are not always available. We, instead, use a combination of solution NMR epitope mapping and ...

Università della Svizzera italiana

Memory T cells in latent mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset

Lindestam Arlehamn, Cecilia S. ; Gerasimova, Anna ; Mele, Federico ; Henderson, Ryan ; Swann, Justine ; Greenbaum, Jason A. ; Kim, Yohan ; Sidney, John ; James, Eddie A. ; Taplitz, Randy ; McKinney, Denise M. ; Kwok, William W. ; Grey, Howard ; Sallusto, Federica ; Peters, Bjoern ; Sette, Alessandro

In: Plos pathogens, 2013, vol. 9, no. 1, p. e1003130

An understanding of the immunological footprint of Mycobacterium tuberculosis (MTB) CD4 T cell recognition is still incomplete. Here we report that human Th1 cells specific for MTB are largely contained in a CXCR3+CCR6+ memory subset and highly focused on three broadly immunodominant antigenic islands, all related to bacterial secretion systems. Our results refute the notion that secreted...

Università della Svizzera italiana

Pemphigus autoantibodies generated through somatic mutations target the desmoglein-3 cis-interface

Di Zenzo, Giovanni ; Di Lullo, Giulia ; Corti, Davide ; Calabresi, Valentina ; Sinistro, Anna ; Vanzetta, Fabrizia ; Didona, Biagio ; Cianchini, Giuseppe ; Hertl, Michael ; Eming, Rudiger ; Amagai, Masayuki ; Ohyama, Bungo ; Hashimoto, Takashi ; Sloostra, Jerry ; Sallusto, Federica ; Zambruno, Giovanna ; Lanzavecchia, Antonio

In: The journal of clinical investigation, 2012, vol. 122, no. 10, p. 3781-3790

Pemphigus vulgaris (PV) is an autoimmune blistering disease of skin and mucous membranes caused by autoantibodies to the desmoglein (DSG) family proteins DSG3 and DSG1, leading to loss of keratinocyte cell adhesion. To learn more about pathogenic PV autoantibodies, we isolated 15 IgG antibodies specific for DSG3 from 2 PV patients. Three antibodies disrupted keratinocyte monolayers in vitro,...

Università della Svizzera italiana

Systems analysis of MVA-C induced immune response reveals its significance as a vaccine candidate against HIV/AIDS of clade C

Gómez, Carmen Elena ; Perdiguero, Beatriz ; Jiménez, Victoria ; Filali-Mouhim, Abdelali ; Ghneim, Khader ; Haddad, Elias K. ; Quakkerlaar, Esther D. ; Delaloye, Julie ; Harari, Alexandre ; Roger, Thierry ; Dunhen, Thomas ; Sékaly, Rafick P. ; Melief, Cornelis J. M. ; Calandra, Thierry ; Sallusto, Federica ; Lanzavecchia, Antonio ; Wagner, Ralf ; Pantaleo, Giuseppe ; Esteban, Mariano

In: Plos one, 2012, vol. 7, no. 4, p. e35485

Based on the partial efficacy of the HIV/AIDS Thai trial (RV144) with a canarypox vector prime and protein boost, attenuated poxvirus recombinants expressing HIV-1 antigens are increasingly sought as vaccine candidates against HIV/AIDS. Here we describe using systems analysis the biological and immunological characteristics of the attenuated vaccinia virus Ankara strain expressing the HIV-1...

Università della Svizzera italiana

T regulatory cells are markers of disease activity in multiple sclerosis patients

Dalla Libera, Dacia ; Di Mitri, Diletta ; Bergami, Alessandra ; Centonze, Diego ; Gasperini, Claudio ; Grasso, Maria Grazia ; Galgani, Simona ; Martinelli, Vittorio ; Comi, Giancarlo ; Avolio, Carlo ; Martino, Gianvito ; Borsellino, Giovanna ; Sallusto, Federica ; Battistini,Luca ; Furlan, Roberto

In: Plos one, 2011, vol. 6, no. 6, p. e21386

FoxP3+ Treg cells are believed to play a role in the occurrence of autoimmunity and in the determination of clinical recurrences. Contradictory reports are, however, available describing frequency and function of Treg cells during autoimmune diseases. We examined, by both polychromatic flow cytometry, and real-time RT-PCR, several Treg markers in peripheral blood mononuclear cells from...

Università della Svizzera italiana

In-depth analysis of the antibody response of individuals exposed to primary dengue virus infection

Alwis, Ruklanthi de ; Beltramello, Martina ; Messer, William B. ; Sukupolvi-Petty, Soila ; Wahala, Wahala M. P. B. ; Kraus, Annette ; Olivarez, Nicholas P. ; Pham, Quang ; Brian, James ; Tsai, Wen-Yang ; Wang, Wei-Kung ; Halstead, Scott ; Kliks, Srisakul ; Diamond, Michael S. ; Baric, Ralph ; Lanzavecchia, Antonio ; Sallusto, Federica ; Silva, Aravinda M. de

In: Plos neglected tropical diseases, 2011, vol. 5, no. 6, p. e1188

Humans who experience a primary dengue virus (DENV) infection develop antibodies that preferentially neutralize the homologous serotype responsible for infection. Affected individuals also generate cross-reactive antibodies against heterologous DENV serotypes, which are non-neutralizing. Dengue cross-reactive, non-neutralizing antibodies can enhance infection of Fc receptor bearing cells and,...

Università della Svizzera italiana

An anti-HIV-1 V3 loop antibody fully protects cross-clade and elicits T-cell immunity in macaques mucosally challenged with an R5 clade C SHIV

Watkins, Jennifer D. ; Siddappa, Nagadenahalli B. ; Lakhashe, Samir K. ; Humbert, Michael ; Sholukh, Anton ; Hemashettar, Girish ; Wong, Yin Ling ; Yoon, John K. ; Wang, Wendy ; Novembre, Francis J. ; Villinger, Francois ; Ibegbu, Chris ; Patel, Kalpana ; Corti, Davide ; Agatic, Gloria ; Vanzetta, Fabrizio ; Bianchi, Siro ; Heeney, Jonathan L. ; Sallusto, Federica ; Lanzavecchia, Antonio ; Ruprecht, Ruth M.

In: Plos one, 2011, vol. 6, no. 3, p. e18207

Neutralizing antibodies have been shown to protect macaques against SHIV challenge. However, genetically diverse HIV-1 clades have evolved, and a key question left unanswered is whether neutralizing antibodies can confer cross-clade protection in vivo. The novel human monoclonal antibody HGN194 was isolated from an individual infected with an HIV-1 clade AG recombinant circulating recombinant...

Università della Svizzera italiana

Improved innate and adaptive immunostimulation by genetically modified HIV-1 protein expressing NYVAC vectors

Quakkelaar, Esther D. ; Redeker, Anke ; Haddad, Elias K. ; Harari, Alexandre ; McCaughey, Stella Mayo ; Duhen, Thomas ; Filali-Mouhim, Abdelali ; Goulet, Jean-Philippe ; Loof, Nikki M. ; Ossendorp, Ferry ; Perdiguero, Beatriz ; Heinen, Paul ; Gomez, Carmen E. ; Kibler, Karen V. ; Koelle, David M. ; Sékaly, Rafick P. ; Sallusto, Federica ; Lanzavecchia, Antonio ; Pantaleo, Giuseppe ; Esteban, Mariano ; Tartaglia, Jim ; Jacobs, Bertram L. ; Melief, Cornelis J. M.

In: Plos one, 2011, vol. 6, no. 2, p. e16819

Attenuated poxviruses are safe and capable of expressing foreign antigens. Poxviruses are applied in veterinary vaccination and explored as candidate vaccines for humans. However, poxviruses express multiple genes encoding proteins that interfere with components of the innate and adaptive immune response. This manuscript describes two strategies aimed to improve the immunogenicity of the...