Università della Svizzera italiana

Improved innate and adaptive immunostimulation by genetically modified HIV-1 protein expressing NYVAC vectors

Quakkelaar, Esther D. ; Redeker, Anke ; Haddad, Elias K. ; Harari, Alexandre ; McCaughey, Stella Mayo ; Duhen, Thomas ; Filali-Mouhim, Abdelali ; Goulet, Jean-Philippe ; Loof, Nikki M. ; Ossendorp, Ferry ; Perdiguero, Beatriz ; Heinen, Paul ; Gomez, Carmen E. ; Kibler, Karen V. ; Koelle, David M. ; Sékaly, Rafick P. ; Sallusto, Federica ; Lanzavecchia, Antonio ; Pantaleo, Giuseppe ; Esteban, Mariano ; Tartaglia, Jim ; Jacobs, Bertram L. ; Melief, Cornelis J. M.

In: Plos one, 2011, vol. 6, no. 2, p. e16819

Attenuated poxviruses are safe and capable of expressing foreign antigens. Poxviruses are applied in veterinary vaccination and explored as candidate vaccines for humans. However, poxviruses express multiple genes encoding proteins that interfere with components of the innate and adaptive immune response. This manuscript describes two strategies aimed to improve the immunogenicity of the...

Università della Svizzera italiana

An MVA vector expressing HIV-1 envelope under the control of a potent vaccinia virus promoter as a promising strategy in HIV/AIDS vaccine design

Pérez, Patricia ; Marín, María Q. ; Lázaro-Frías, Adrián ; Sorzano, Carlos Óscar S. ; Di Pilato, Mauro ; Gómez, Carmen E. ; Esteban, Mariano ; García-Arriaza, Juan

In: Vaccines, 2019, vol. 7, no. 4, p. 208

Highly attenuated poxviral vectors, such as modified vaccinia virus ankara (MVA), are promising vaccine candidates against several infectious diseases. One of the approaches developed to enhance the immunogenicity of poxvirus vectors is increasing the promoter strength and accelerating during infection production levels of heterologous antigens. Here, we have generated and characterized the ...

Università della Svizzera italiana

Systems analysis of MVA-C induced immune response reveals its significance as a vaccine candidate against HIV/AIDS of clade C

Gómez, Carmen Elena ; Perdiguero, Beatriz ; Jiménez, Victoria ; Filali-Mouhim, Abdelali ; Ghneim, Khader ; Haddad, Elias K. ; Quakkerlaar, Esther D. ; Delaloye, Julie ; Harari, Alexandre ; Roger, Thierry ; Dunhen, Thomas ; Sékaly, Rafick P. ; Melief, Cornelis J. M. ; Calandra, Thierry ; Sallusto, Federica ; Lanzavecchia, Antonio ; Wagner, Ralf ; Pantaleo, Giuseppe ; Esteban, Mariano

In: Plos one, 2012, vol. 7, no. 4, p. e35485

Based on the partial efficacy of the HIV/AIDS Thai trial (RV144) with a canarypox vector prime and protein boost, attenuated poxvirus recombinants expressing HIV-1 antigens are increasingly sought as vaccine candidates against HIV/AIDS. Here we describe using systems analysis the biological and immunological characteristics of the attenuated vaccinia virus Ankara strain expressing the HIV-1...