Université de Fribourg

Quantification of gold nanoparticle cell uptake under controlled biological conditions and adequate resolution

Rothen-Rutishauser, Barbara ; Kuhn, Dagmar A ; Ali, Zulqurnain ; Gasser, Michael ; Amin, Faheem ; Parak, Wolfgang J ; Vanhecke, Dimitri ; Fink, Alke ; Gehr, Peter ; Brandenberger, Christina

In: Nanomedicine, 2014, vol. 9, no. 5, p. 607–621

Aim: We examined cellular uptake mechanisms of fluorescently labeled polymer-coated gold nanoparticles (NPs) under different biological conditions by two quantitative, microscopic approaches. Materials & methods: Uptake mechanisms were evaluated using endocytotic inhibitors that were tested for specificity and cytotoxicity. Cellular uptake of gold NPs was analyzed either by laser scanning...