Université de Fribourg

Biodistribution, clearance, and long‐term fate of clinically relevant nanomaterials

Bourquin, Joël ; Milosevic, Ana ; Hauser, Daniel ; Lehner, Roman ; Blank, Fabian ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Advanced Materials, 2018, p. -

Realization of the immense potential of nanomaterials for biomedical applications will require a thorough understanding of how they interact with cells, tissues, and organs. There is evidence that, depending on their physicochemical properties and subsequent interactions, nanomaterials are indeed taken up by cells. However, the subsequent release and/or intracellular degradation of the...

Université de Fribourg

Aerosol delivery of functionalized gold nanoparticles target and activate dendritic cells in a 3D lung cellular model

Fytianos, Kleanthis ; Chortarea, Savvina ; Rodriguez-Lorenzo, Laura ; Blank, Fabian ; Garnier, Christophe von ; Petri-Fink, Alke ; Rothen-Rutishauser, and Barbara

In: ACS Nano, 2017, vol. 11, no. 1, p. 375–383

Nanocarrier design combined with pulmonary drug delivery holds great promise for the treatment of respiratory tract disorders. In particular, targeting of dendritic cells that are key immune cells to enhance or suppress an immune response in the lung is a promising approach for the treatment of allergic diseases. Fluorescently encoded poly(vinyl alcohol) (PVA)-coated gold nanoparticles,...

Université de Fribourg

Interaction of biomedical nanoparticles with the pulmonary immune system

Blank, Fabian ; Fytianos, Kleanthis ; Seydoux, Emilie ; Rodriguez-Lorenzo, Laura ; Petri-Fink, Alke ; Garnier, Christophe von ; Rothen-Rutishauser, Barbara

In: Journal of Nanobiotechnology, 2017, vol. 15, p. 6

Engineered nanoparticles (NPs) offer site-specific delivery, deposition and cellular uptake due to their unique physicochemical properties and were shown to modulate immune responses. The respiratory tract with its vast surface area is an attractive target organ for innovative immunomodulatory therapeutic applications by pulmonary administration of such NPs, enabling interactions with...

Université de Fribourg

Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro

Fytianos, Kleanthis ; Rodriguez-Lorenzo, Laura ; Clift, Martin J.D. ; Blank, Fabian ; Vanhecke, Dimitri ; Garnier, Christophe von ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Nanomedicine: Nanotechnology, Biology and Medicine, 2015, vol. 11, no. 3, p. 633–644

Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical...

Université de Fribourg

Engineering an in vitro air-blood barrier by 3D bioprinting

Horváth, Lenke ; Umehara, Yuki ; Jud, Corinne ; Blank, Fabian ; Petri-Fink, Alke ; Rothen-Rutishauser, Barbara

In: Scientific Reports, 2015, vol. 5, p. -

Intensive efforts in recent years to develop and commercialize in vitro alternatives in the field of risk assessment have yielded new promising two- and three dimensional (3D) cell culture models. Nevertheless, a realistic 3D in vitro alveolar model is not available yet. Here we report on the biofabrication of the human air-blood tissue barrier analogue composed of an endothelial cell, basement...

Université de Fribourg

Size-dependent accumulation of particles in lysosomes modulates dendritic cell function through impaired antigen degradation

Seydoux, Emilie ; Rothen-Rutishauser, Barbara ; Nita, Izabela M ; Balog, Sandor ; Gazdhar, Amiq ; Stumbles, Philip A ; Petri-Fink, Alke ; Blank, Fabian ; Garnier, Christophe von

In: International Journal of Nanomedicine, 2014, p. 3885

Introduction: Nanosized particles may enable therapeutic modulation of immune responses by targeting dendritic cell (DC) networks in accessible organs such as the lung. To date, however, the effects of nanoparticles on DC function and downstream immune responses remain poorly understood. Methods: Bone marrow–derived DCs (BMDCs) were exposed in vitro to 20 or...

Université de Fribourg

Fluorescence-encoded gold nanoparticles: library design and modulation of cellular uptake into dendritic cells

Rodriguez-Lorenzo, Laura ; Fytianos, Kleanthis ; Blank, Fabian ; Garnier, Christophe von ; Rothen-Rutishauser, Barbara ; Petri-Fink, Alke

In: Small, 2014, vol. 10, no. 7, p. 1341–1350

In order to harness the unique properties of nanoparticles for novel clinical applications and to modulate their uptake into specific immune cells we designed a new library of homo- and hetero-functional fluorescence-encoded gold nanoparticles (Au-NPs) using different poly(vinyl alcohol) and poly(ethylene glycol)-based polymers for particle coating and stabilization. The encoded particles were...

Université de Fribourg

Biomedical nanoparticles modulate specific CD4+ T cell stimulation by inhibition of antigen processing in dendritic cells

Blank, Fabian ; Gerber, Peter ; Rothen-Rutishauser, Barbara ; Sakulkhu, Usawadee ; Salaklang, Jatuporn ; Peyer, Karin De ; Gehr, Peter ; Nicod, Laurent P. ; Hofmann, Heinrich ; Geiser, Thomas ; Petri-Fink, Alke ; Garnier, Christophe Von

In: Nanotoxicology, 2011, p. -

Understanding how nanoparticles may affect immune responses is an essential prerequisite to developing novel clinical applications. To investigate nanoparticle-dependent outcomes on immune responses, dendritic cells (DCs) were treated with model biomedical poly(vinylalcohol)-coated super-paramagnetic iron oxide nanoparticles (PVA-SPIONs). PVA-SPIONs uptake by human monocyte-derived DCs (MDDCs)...