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Université de Fribourg

Functional characterization of a miniature inverted transposable element at the origin of mcr-5 gene acquisition in Escherichia coli

Kieffer, Nicolas ; Nordmann, Patrice ; Millemann, Yves ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2019/63/7/e00559-19, /aac/63/7/AAC.00559-19.atom

Plasmid-mediated colistin resistance of the mobile colistin resistance (MCR) type is a growing concern in Enterobacteriaceae since it has been described worldwide in humans and animals. Here, we identified a series of MCR-producing Escherichia coli isolates corresponding to two different clones (represented by isolates PS1 and PS8b) producing MCR-1 and MCR-5, respectively, obtained from pig...

Université de Fribourg

Full genome sequence of pT3, a multiresistant plasmid carrying the mcr-3.5 colistin resistance gene, recovered from an extended-spectrum-β-lactamase-producing Escherichia coli isolate from crickets sold as food

Zurfluh, Katrin ; Stevens, Marc J. A. ; Bucher, Melissa ; Poirel, Laurent ; Nordmann, Patrice ; Stephan, Roger

In: Microbiology Resource Announcements, 2019/8/29/e00647-19, /mra/8/29/MRA.00647-19.atom

Here, we sequenced multidrug resistance plasmid pT3, which encodes mcr-3.5 and other resistance genes. The cooccurrence of mcr genes and other resistance genes on a single plasmid is of concern.

Université de Fribourg

ESBLs and resistance to ceftazidime/avibactam and ceftolozane/tazobactam combinations in Escherichia coli and Pseudomonas aeruginosa

Ortiz de la Rosa, José-Manuel ; Nordmann, Patrice ; Poirel, Laurent

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 7, p. 1934–1939

To evaluate the efficacy of the recently launched β-lactam/β-lactamase inhibitor combinations ceftazidime/avibactam and ceftolozane/tazobactam against ESBL- producing Escherichia coli and Pseudomonas aeruginosa strains.Methods: A series of ESBL-encoding genes (blaTEM, blaSHV, blaCTX-M, blaVEB, blaPER, blaGES and blaBEL) was cloned and expressed in E. coli or P. aeruginosa recipient strains. ...

Université de Fribourg

Characterization of PAN-1, a carbapenem-hydrolyzing class b β-lactamase from the environmental gram-negative Pseudobacteriovorax antillogorgiicola

Kieffer, Nicolas ; Poirel, Laurent ; Fournier, Claudine ; Haltli, Brad ; Kerr, Russel ; Nordmann, Patrice

In: Frontiers in Microbiology, 2019, vol. 10, p. -

The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species Pseudobacteriovorax antillogorgiicola. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM- 1, its closest relative. Kinetic parameters performed on purified PAN-1 showed it displayed a hydrolytic activity toward most β-lactams including carbapenems but ...

Université de Fribourg

Colistin resistance in Parisian inpatient faecal Escherichia coli as the result of two distinct evolutionary pathways

Bourrel, Anne Sophie ; Poirel, Laurent ; Royer, Guilhem ; Darty, Mélanie ; Vuillemin, Xavier ; Kieffer, Nicolas ; Clermont, Olivier ; Denamur, Erick ; Nordmann, Patrice ; Decousser, Jean-Winoc ; The IAME Resistance Group

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 6, p. 1521–1530

Beyond plasmid-encoded resistance (mcr genes) prevalence in strain collections, large epidemiological studies to estimate the human burden of colistin-resistant Escherichia coli gut carriage are lacking.Objectives: To evaluate the prevalence of colistin-resistant E. coli carriage in inpatients and decipher the molecular support of resistance and the genetic background of the strains.Methods:...

Université de Fribourg

A resazurin reduction-based assay for rapid detection of polymyxin resistance in Acinetobacter baumannii and Pseudomonas aeruginosa

Lescat, Mathilde ; Poirel, Laurent ; Tinguely, Camille ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2018, p. -

A rapid test was developed for identification of polymyxin resistance in non-fermenting bacteria. This test detects viable cells after growth in a medium containing a defined concentration of colistin. The principle of this test is based on the visual detection of the reduction of the resazurin reagent, a viability colorant, observed by its color change (blue to purple or pink). Its...

Université de Fribourg

Screening for Intestinal Carriage of Extended-spectrum Beta-lactamase–producing Enterobacteriaceae in Critically Ill Patients: Expected Benefits and Evidence-based Controversies

Zahar, Jean-Ralph ; Blot, Stijn ; Nordmann, Patrice ; Martischang, Romain ; Timsit, Jean-François ; Harbarth, Stephan ; Barbier, François

In: Clinical Infectious Diseases, 2018, p. -

The rising burden of intensive care unit (ICU)-acquired infections due to extended- spectrum beta-lactamase–producing Enterobacteriaceae (ESBL-E) strengthens the requirement for efficient prevention strategies. The detection of intestinal carriage of ESBL-E through active surveillance cultures (ASC) and the implementation of contact precautions (CP) in carriers are currently advocated in...

Université de Fribourg

Rapid detection of fosfomycin resistance in Escherichia coli

Nordmann, Patrice ; Poirel, Laurent ; Mueller, Linda

In: Journal of Clinical Microbiology, 2019, vol. 57, no. 1, p. e01531-18

The rapid fosfomycin/Escherichia coli NP test was developed to detect fosfomycin resistance in E. coli isolates. The test is based on glucose metabolization and the detection of bacterial growth in the presence of fosfomycin at 40 µg/ml. Bacterial growth is visually detectable by an orange-to-yellow color change of red phenol, a pH indicator. A total of 100 E. coli isolates, among which 22...