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Université de Fribourg

Epidemiology of Carbapenemase-Producing Klebsiella pneumoniae in a Hospital, Portugal

Aires-de-Sousa, Marta ; Ortiz de la Rosa, José Manuel ; Gonçalves, Maria Luísa ; Pereira, Ana Luísa ; Nordmann, Patrice ; Poirel, Laurent

In: Emerging Infectious Diseases, 2019, vol. 25, no. 9, p. 1632–1638

We aimed to provide updated epidemiologic data on carbapenem-resistant Klebsiella pneumoniae in Portugal by characterizing all isolates (N = 46) recovered during 2013– 2018 in a 123-bed hospital in Lisbon. We identified blaKPC-3 (n = 36), blaOXA-181 (n = 9), and blaGES-5 (n = 8) carbapenemase genes and observed co-occurrence of blaKPC-3 and blaGES-5 in 7 isolates. A single GES-5–producing...

Université de Fribourg

Antimicrobial activity of octenidine against multidrug-resistant Gram-negative pathogens

Alvarez-Marin, R. ; Aires-de-Sousa, Marta ; Nordmann, Patrice ; Kieffer, Nicolas ; Poirel, Laurent

In: European Journal of Clinical Microbiology & Infectious Diseases, 2017, p. 1–5

Multidrug-resistant (MR) Gram-negative (GN) pathogens pose a major and growing threat for healthcare systems, as therapy of infections is often limited due to the lack of available systemic antibiotics. Well-tolerated antiseptics, such as octenidine dihydrochloride (OCT), may be a very useful tool in infection control to reduce the dissemination of MRGN. This study aimed to investigate the...

Université de Fribourg

First case of NDM-1 producing Klebsiella pneumoniae in Caribbean islands

Bastian, Sylvaine ; Nordmann, Patrice ; Creton, Elodie ; Malpote, Edith ; Thiery, Guillaume ; Martino, Frederic ; Breurec, Sebastien ; Dortet, Laurent

In: International Journal of Infectious Diseases, 2015, vol. 34, p. 53–54

Characterize a NDM-1 producing K. pneumoniae isolate recovered from a patient hospitalized in Guadeloupe, French West Indies, after its transfer from CubaMethodsAntibiotic susceptibilities were determined by the disk diffusion method, and E-test. Carbapenemase production was assessed using the Carba NP test. Antibiotic resistance determinants and their surrounding structures were...

Université de Fribourg

In vitro prediction of the evolution of the GES-1 β-lactamase hydrolytic activity

Bontron, Séverine ; Poirel, Laurent ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2015, p. AAC.04450–14

Resistance to ß-lactams is constantly increasing, due to the emergence of totally new enzymes, but also to the evolution of pre-existing ß-lactamases. GES-1 is a clinically-relevant extended-spectrum β-lactamase (ESBL) hydrolyzing penicillins and broad-spectrum cephalosporins, but sparing monobactams and carbapenems. However, several GES-1 variants (i.e. GES-2 and GES-5) previously identified...

Université de Fribourg

Transposition of tn125 encoding the ndm-1 carbapenemase in Acinetobacter baumannii

Bontron, Séverine ; Nordmann, Patrice ; Poirel, Laurent

In: Antimicrobial Agents and Chemotherapy, 2016, vol. 60, no. 12, p. 7245–7251

The blaNDM-1 gene encodes a carbapenemase that confers resistance to almost all β-lactams, including last-resort carbapenems. This is increasingly reported worldwide in nosocomial and community-acquired Gram-negative bacteria. Acinetobacter baumannii is an important opportunistic pathogen that is considered an intermediate reservoir for the blaNDM-1 gene. In this species, the blaNDM-1 gene...

Université de Fribourg

Real-time PCR for detection of plasmid-mediated polymyxin resistance (mcr-1) from cultured bacteria and stools

Bontron, Séverine ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2016, p. dkw139

The aim of the study was to develop a simple assay for rapid detection of the mcr-1 gene, recently identified as a source of plasmid-mediated acquired resistance to polymyxins in Enterobacteriaceae.Methods: A SYBR Green-based real-time PCR assay was designed for detection of the mcr-1 gene. This assay was applied to cultured bacteria and to spiked human and cattle stools.Results: The mcr-1...

Université de Fribourg

Crystal Structure of DIM-1, an Acquired Subclass B1 Metallo-β-Lactamase from Pseudomonas stutzeri

Booth, Michael P. S. ; Kosmopoulou, Magda ; Poirel, Laurent ; Nordmann, Patrice ; Spencer, James

In: PLoS ONE, 2015, vol. 10, no. 10, p. e0140059

Metallo-β-lactamases (MBLs) hydrolyze almost all classes of β-lactam antibiotic, including carbapenems—currently first choice drugs for opportunistic infections by Gram-negative bacterial pathogens. MBL inhibitor development is complicated by the diversity within this group of enzymes, and by the appearance of new enzymes that continue to be identified both as chromosomal genes and on mobile...

Université de Fribourg

Integrase-mediated recombination of the bel-1 gene cassette encoding the extended-spectrum β-lactamase BEL-1

Bouheraoua, Nacim ; Poirel, Laurent ; Bourreau, Baptiste ; Bonnin, Rémy ; Laroche, Laurent ; Naas, Thierry ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2018, p. AAC.00030-18

Integrons are genetic elements that can acquire and rearrange gene cassettes. The blaBEL-1 gene encodes an extended-spectrum β-lactamase, BEL-1, that is present at second position of the variable region of class 1 integrons identified in Pseudomonas aeruginosa. The mobility of the bel-1 gene cassette was analyzed in physiological conditions and with the integrase gene being overexpressed....

Université de Fribourg

Colistin resistance in Parisian inpatient faecal Escherichia coli as the result of two distinct evolutionary pathways

Bourrel, Anne Sophie ; Poirel, Laurent ; Royer, Guilhem ; Darty, Mélanie ; Vuillemin, Xavier ; Kieffer, Nicolas ; Clermont, Olivier ; Denamur, Erick ; Nordmann, Patrice ; Decousser, Jean-Winoc ; The IAME Resistance Group

In: Journal of Antimicrobial Chemotherapy, 2019, vol. 74, no. 6, p. 1521–1530

Beyond plasmid-encoded resistance (mcr genes) prevalence in strain collections, large epidemiological studies to estimate the human burden of colistin-resistant Escherichia coli gut carriage are lacking.Objectives: To evaluate the prevalence of colistin-resistant E. coli carriage in inpatients and decipher the molecular support of resistance and the genetic background of the strains.Methods:...