Université de Fribourg

Analysis of OXA-204 carbapenemase-producing Enterobacteriaceae reveals possible endoscopy-associated transmission, France, 2012 to 2014

Potron, Ana?s ; Bernabeu, Sandrine ; Cuzon, Gaëlle ; Ponti?s, Valérie ; Blanchard, Hervé ; Seringe, Elise ; Naas, Thierry ; Nordmann, Patrice ; Dortet, Laurent

In: Eurosurveillance, 2017, vol. 22, no. 49, p. 17-00048

OXA-48-like beta-lactamase producing bacteria are now endemic in several European and Mediterranean countries. Among this carbapenemase family, the OXA-48 and OXA-181 variants predominate, whereas other variants such as OXA-204 are rarely reported. Here, we report the molecular epidemiology of a collection of OXA-204- positive enterobacterial isolates (n = 29) recovered in France between...

Université de Fribourg

Trends in carbapenemase-producing Enterobacteriaceae, France, 2012 to 2014

Dortet, Laurent ; Cuzon, Gaëlle ; Ponties, V ; Nordmann, Patrice

In: Eurosurveillance, 2017, vol. 22, no. 6, p. -

In 2014, a total of 2,976 Enterobacteriaceae isolates with decreased susceptibility to carbapenems were received at the French Associated National Reference Center for Antibiotic Resistance (NRC) and were characterised for their molecular resistance mechanism to carbapenems and compared with results obtained during 2012 and 2013.The overall number of enterobacterial isolates with decreased...

Université de Fribourg

Neonatal infections with multidrug-resistant ESBL-producing E. cloacae and K. pneumoniae in Neonatal Units of two different Hospitals in Antananarivo, Madagascar

Naas, Thierry ; Cuzon, Gaëlle ; Robinson, A. L. ; Andrianirina, Z. ; Imbert, P. ; Ratsima, E. ; Ranosiarisoa, Z. N. ; Nordmann, Patrice ; Raymond, J.

In: BMC Infectious Diseases, 2016, vol. 16, p. 275

Background: We investigated the molecular mechanism of ß-lactam resistance in extended-spectrum ß-lactamase (ESBL)-producing Enterobacterial strains isolated in neonatal units of different hospitals in Anatnanarivo, Madagascar.Methods: Bacteria were identified by standard biochemical methods, disc diffusion antibiograms and Etest. Resistance genes were sought by PCR. Strains were...

Université de Fribourg

Evaluation of the RAPIDEC® CARBA NP, the Rapid CARB Screen® and the Carba NP test for biochemical detection of carbapenemase-producing Enterobacteriaceae

Dortet, Laurent ; Agathine, Aurélie ; Naas, Thierry ; Cuzon, Gaëlle ; Poirel, Laurent ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2015, vol. 70, no. 11, p. 3014-3022

The objective of this study was the evaluation of the performance of two commercially available biochemical tests for the rapid detection of carbapenemase-producing Enterobacteriaceae compared with a home-made technique.Methods: A collection of 150 enterobacterial isolates, including 132 isolates with decreased susceptibility to at least one carbapenem molecule, were tested for carbapenemase...

Université de Fribourg

Multiple colonization with highly resistant bacteria: carbapenemase-producing Enterobacteriaceae, carbapenemase-producing Pseudomonas aeruginosa, carbapenemase-producing Acinetobacter baumannii, and glycopeptide-resistant Enterococcus faecium

Girlich, Delphine ; Nordmann, Patrice ; Lécuyer, Hervé ; Berche, Patrick ; Marmorat-Khuong, Anne ; Gros, Isabelle ; Cuzon, Gaëlle ; Dortet, Laurent

In: Diagnostic Microbiology and Infectious Disease, 2015, p. -

The dissemination of carbapenemase-producing bacteria worldwide is an important source of concern because carbapenemase producers are multidrug resistant (Nordmann and Poirel, 2014). National guidelines increasingly recommend a systematic screening of at least carbapenemase-producing Enterobacteriaceae (CPE) and glycopeptide-resistant enterococci (GRE) in patients admitted to hospitals who have...

Université de Fribourg

Strategy for a rapid detection of carbapenemase-producing Enterobacteriaceae

Dortet, Laurent ; Bréchard, Ludivine ; Cuzon, Gaëlle ; Poirel, Laurent ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2014, vol. 58, no. 4, p. 2441-2445

A prospective survey was conducted on 862 Enterobacteriaceae with reduced susceptibility to carbapenems. The Carba NP test, UV spectrophotometry and DNA microarray were used for detecting carbapenemase producers, and results compared to PCR and sequencing. The 172 carbapenemase producers were detected using the Carba NP test and UV spectrophotometry whereas DNA microarray failed to detect IMI...