Université de Neuchâtel

Performance of an Integrated Microoptical System for Fluorescence Detection in Microfluidic Systems

Roulet , Jean-Christophe ; Völkel, Reinhard ; Herzig, Hans Peter ; Verpoorte, Elisabeth ; de Rooij, Nicolaas F. ; Dändliker, René

In: Analytical Chemistry, 2020, vol. 74, no. 14, p. 3400-3407

This article presents a new integrated microfluidic/microoptic device designed for basic biochemical analysis. The microfluidic network is wet-etched in a Borofloat 33 (Pyrex) glass wafer and sealed by means of a second wafer. Unlike other similar microfluidic systems, elements of the detection system are realized with the help of microfabrication techniques and directly deposited on both sides...

Université de Neuchâtel

Microfluidics/CMOS orthogonal capabilities for cell biology

Linder, Vincent ; Koster, Sander ; Franks, Wendy ; Kraus, Tobias ; Verpoorte, Elisabeth ; Heer, Flavio ; Hierlemann, Andreas ; de Rooij, Nicolaas F.

In: Biomedical Microdevices, 2006, vol. 8, no. 2, p. 159-166

The study of individual cells and cellular networks can greatly benefit from the capabilities of microfabricated devices for the stimulation and the recording of electrical cellular events. In this contribution, we describe the development of a device, which combines capabilities for both electrical and pharmacological cell stimulation, and the subsequent recording of electrical cellular...

Université de Neuchâtel

Multi-layer microfluidic glass chips for microanalytical applications

Daridon, Antoine ; Fascio, Valia ; Lichtenberg, Jan ; Wütrich, Rolf ; Langen, Hans ; Verpoorte, Elisabeth ; de Rooij, Nicolaas F.

In: Fresenius' Journal of Analytical Chemistry, 2001, vol. 371, no. 2, p. 261-269

A new, versatile architecture is presented for microfluidic devices made entirely from glass, for use with reagents which would prove highly corrosive for silicon. Chips consist of three layers of glass wafers bonded together by fusion bonding. On the inside wafer faces a network of microfluidic channels is created by photolithography and wet chemical etching. Low dead-volume fluidic connections...

Université de Neuchâtel

Microlens systems for fluorescence detection in chemical microsystems

Roulet, Jean-Christophe ; Völkel, Reinhard ; Herzig, Hans-Peter ; Verpoorte, Elisabeth ; de Rooij, Nicolaas F. ; Dändliker, René

In: Optical Engineering, 2001, vol. 40, no. 5, p. 814-821

Micro-optical systems based on refractive microlenses are investigated. These systems are integrated on a chemical chip. They focus an excitation beam into the detection volume (microliter or even submicroliter scale) and collect the emitted light from fluorescent molecules. The fluorescence must be carefully separated by spatial and spectral filtering from the excitation. This paper presents the...