The Mi-2 nucleosome-remodeling protein LET-418 is targeted via LIN-1/ETS to the promoter of lin-39/Hox during vulval development in C. elegans
- Guerry, Frédéric Département de Biologie, Université de Fribourg, Switzerland
- Marti, Claude-Olivier Département de Biologie, Université de Fribourg, Switzerland
- Zhang, Yue Département de Biologie, Université de Fribourg, Switzerland
- Moroni, Paolo S. Département de Biologie, Université de Fribourg, Switzerland
- Jaquiéry, Emilie Département de Biologie, Université de Fribourg, Switzerland
- Müller, Fritz Département de Biologie, Université de Fribourg, Switzerland
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24.03.2007
Published in:
- Developmental Biology. - 2007, vol. 206, no. 2, p. 469-479
English
The fate of the vulval cells in Caenorhabditis elegans is specified, at least in part, through a highly conserved RTK/Ras mediated signaling cascade that negatively regulates the activity of the ETS-like transcription factor LIN-1. The Hox gene lin-39 functions downstream of both, the LIN-3/RTK/Ras pathway and LIN-1 and plays a pivotal role in controlling vulva cell competence and induction. Here we show that LET-418, a C. elegans ortholog of the human NuRD component Mi-2, negatively modulates the activity of lin-39. LET-418 interacts in vivo with specific regions in the promoter of lin-39 and this interaction depends on LIN-1. Our data provide evidence for a model in which LIN-1 recruits LET-418/Mi-2 as co-repressor to the promoter of lin-39, thereby restricting its activity to the basal levels required in the vulva precursor cells (VPCs) for normal vulval development. Thus, our data suggest that the interaction between LIN-1 and LET-418/Mi-2 may link RTK/Ras signaling with chromatin remodeling and gene expression.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 8651
- DOI 10.1016/j.ydbio.2007.03.026
- Persistent URL
- https://folia.unifr.ch/unifr/documents/300449
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