Faculté des sciences

Microfabrication and characterization of an ion-selective microelectrode array platform

Guenat, Olivier T. ; Dufour, J.-F. ; van der Wal, P.D. ; Morf, Werner E. ; de Rooij, Nicolaas F. ; Koudelka-Hep, Milena

In: Sensors and Actuators B : Chemical, 2005, vol. 105, no. 1, p. 65-73

The design and the fabrication of an ion-selective electrode array platform aimed at in vitro intracellular recording are presented. The platform is composed of two parts: (i) a glass chip with microchannels whose bottom part is patterned with a platinum layer and (ii) a silicon chip in which an array of 50 μm long silicon nitride micropipettes with an inner diameter of 5 μm are structured. The... More

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    Summary
    The design and the fabrication of an ion-selective electrode array platform aimed at in vitro intracellular recording are presented. The platform is composed of two parts: (i) a glass chip with microchannels whose bottom part is patterned with a platinum layer and (ii) a silicon chip in which an array of 50 μm long silicon nitride micropipettes with an inner diameter of 5 μm are structured. The 24 micropipettes are individually filled with a Ca2+ selective membrane, based on the neutral ionophore ETH 129. Preliminary tests in Ca2+ buffered solutions have shown a linear range from 10−6 to 0.1 M Ca2+ with a sensitivity of 27 mV/decade.