Faculté des sciences

High-density electrode array for imaging in vitro electrophysiological activity

Berdondini, Luca ; van der Wal, P.D. ; Guenat, Olivier ; de Rooij, Nicolaas F. ; Koudelka-Hep, Milena ; Seitz, P. ; Kaufmann, R. ; Metzler, P. ; Blanc, N. ; Rohr, S.

In: Biosensors and Bioelectronics, 2005, vol. 21, no. 1, p. 167

The development of a high-density active microelectrode array for in vitro electrophysiology is reported. Based on the Active Pixel Sensor (APS) concept, the array integrates 4096 gold microelectrodes (electrode separation 20 μm) on a surface of 2.5 mm × 2.5 mm as well as a high-speed random addressing logic allowing the sequential selection of the measuring pixels. Following the electrical... Plus

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    Summary
    The development of a high-density active microelectrode array for in vitro electrophysiology is reported. Based on the Active Pixel Sensor (APS) concept, the array integrates 4096 gold microelectrodes (electrode separation 20 μm) on a surface of 2.5 mm × 2.5 mm as well as a high-speed random addressing logic allowing the sequential selection of the measuring pixels. Following the electrical characterization in a phosphate solution, the functional evaluation has been carried out by recording the spontaneous electrical activity of neonatal rat cardiomyocytes. Signals with amplitudes from 130 μVp-p to 300 μVp-p could be recorded from different pixels. The results demonstrate the suitability of the APS concept for developing a new generation of high-resolution extracellular recording devices for in vitro electrophysiology.