Faculté des sciences

Isolation and characterization of polymorphic microsatellite loci in Acanthoscelides obvelatus Say (Coleoptera: Bruchidae)

Alvarez, Nadir ; Born, Céline ; Risterucci, A.-M. ; Sourrouille, P. ; Benrey, Betty ; Hossaert-McKey, Martine

In: Molecular Ecology Notes, 2004, vol. 4, no. 4, p. 683

Six microsatellite loci were isolated from the bruchid Acanthoscelides obtectus Say (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from 3 to 18. We found high levels of within-population variation at most loci, with heterozygosities ranging from 0 to 0.75. Cross-species amplification of these loci was tested in two other species of the genus... More

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    Summary
    Six microsatellite loci were isolated from the bruchid Acanthoscelides obtectus Say (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from 3 to 18. We found high levels of within-population variation at most loci, with heterozygosities ranging from 0 to 0.75. Cross-species amplification of these loci was tested in two other species of the genus Acanthoscelides, A. obvelatus Bridwell and A. argillaceus Sharp.