Faculté des sciences

Isolation and characterization of polymorphic microsatellite loci in Acanthoscelides obvelatus Bridwell (Coleoptera: Bruchidae)

Alvarez, Nadir ; Aebi, Alexandre ; Risterucci, A.-M. ; Hossaert-McKey, Martine ; Benrey, Betty

In: Molecular Ecology Notes, 2003, vol. 3, no. 1, p. 12-14

Five microsatellite loci were isolated from the bruchid Acanthoscelides obvelatus Bridwell (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from two to 15. We found high levels of within-population variation at most loci, with heterozygosity ranging from 0.182 to 0.900. Cross-species amplification of these loci was tested in two other species of the... More

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    Summary
    Five microsatellite loci were isolated from the bruchid Acanthoscelides obvelatus Bridwell (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from two to 15. We found high levels of within-population variation at most loci, with heterozygosity ranging from 0.182 to 0.900. Cross-species amplification of these loci was tested in two other species of the genus Acanthoscelides, A. obtectus Say and A. argillaceus Sharp.