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Journal article

Induction of (1→3,1→4)-ß-D-Glucan hydrolases in leaves of dark-incubated barley seedlings

  • Roulin, Samuel Institute of Plant Sciences, University of Bern, Switzerland - Swiss Federal Office of Public Health, Biosafety Unit, Bern, Switzerland
  • Buchala, Antony J. Department of Biology, University of Fribourg, Switzerland
  • Fincher, Geoffrey B. Department of Plant Science, University of Adelaide, Australia
    26.01.2002
Published in:
  • Planta. - 2002, vol. 215, no. 1, p. 51-59
Cell wall
Dark induction
(1→3,1→4)-β-D-Glucan
(1→3,1→4)-β-D-Glucan endohydrolase
β-D-Glucan glucohydrolase
Hordeum (cell wall)
English When seedlings of barley (Hordeum vulgare L.) were transferred from a natural light/dark cycle into darkness, (1M3,1M4)-#-D-glucan endohydrolase (EC 3.2.1.73) activity in leaf extracts increased 3- to 4-fold after 2 days. Activity decreased to normal levels within a day if the light/dark cycle was restored. Although there are two (1M3,1M4)-#-D-glucan endohydrolase isoenzymes in barley, the increased enzyme activity in dark-grown seedlings was attributable entirely to increases in isoenzyme EI. Northern hybridization analyses confirmed that mRNA transcripts encoding (1M3,1M4)-#-D-glucan endohydrolase isoenzyme EI accumulated in the leaves of dark-incubated seedlings; no isoenzyme EII mRNA was detected. Activity of #-D-glucan glucohydrolases also increased 10-fold after 2 days of dark treatment. The latter, broad-specificity enzymes release glucose from (1M3,1M4)-#-D-glucans and from #-D-oligoglucosides released by (1M3,1M4)-#-D-glucan endohydrolases. Consistent with the activity patterns of these enzymes, the (1M3,1M4)-#-D-glucan content of leaf cell walls decreased by about 30% when barley seedlings were transferred into darkness. Soluble sugars in the leaves decreased by about 60% during the same period. Because no measurable leaf elongation was detected during the various light/dark treatments, the enzymes were unlikely to be participating in wall loosening and cell elongation. Instead, the results suggest that cell wall (1M3,1M4)-#-D-glucans can be re- mobilized in the non-elongating, dark-incubated leaves and the glucose so generated could serve as an energy source under conditions of sugar depletion.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/299982
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