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Journal article

Identification and biophysical characterization of a very-long-chain-fatty-acid-substituted phosphatidylinositol in yeast subcellular membranes

  • Schneiter, Roger Division of Biochemistry, Department of Medicine, University of Fribourg, Switzerland
  • Brügger, Britta Biochemie-Zentrum der Universität Heidelberg, Universität Heidelberg, Germany
  • Amann, Clare M. Department of Medicinal Chemistry, The University of Utah, Salt Lake City, USA
  • Prestwich, Glenn D. Department of Medicinal Chemistry, The University of Utah, Salt Lake City, USA
  • Epand, Raquel F. Department of Biochemistry, McMaster University, Hamilton, Canada
  • Zellnig, Günther Institute of Plant Physiology, Karl-Franzens University Graz, Austria
  • Wieland, Felix T. Biochemie-Zentrum der Universität Heidelberg, Universität Heidelberg, Germany
  • Epand, Richard M. Department of Biochemistry, McMaster University, Hamilton, Canada
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    27.06.2004
Published in:
  • Biochemical Journal. - 2004, vol. 381, no. 3, p. 941-949
1,2-dielaidoyl-sn-glycero-3-phosphoethanolamine
fatty acid
membrane curvature
nuclear pore complex
subcellular membrane
yeast
English Morphological analysis of a conditional yeast mutant in acetyl-CoA carboxylase acc1ts/mtr7, the rate-limiting enzyme of fatty acid synthesis, suggested that the synthesis of C₂₆ VLCFAs (very-long-chain fatty acids) is important for maintaining the structure and function of the nuclear membrane. To characterize this C₂₆-dependent pathway in more detail, we have now examined cells that are blocked in pathways that require C₂₆. In yeast, ceramide synthesis and remodelling of GPI (glycosylphosphatidylinositol)-anchors are two pathways that incorporate C₂₆ into lipids. Conditional mutants blocked in either ceramide synthesis or the synthesis of GPI anchors do not display the characteristic alterations of the nuclear envelope observed in acc1ts, indicating that the synthesis of another C₂₆-containing lipid may be affected in acc1ts mutant cells. Lipid analysis of isolated nuclear membranes revealed the presence of a novel C₂₆-substituted PI (phosphatidylinositol). This C26-PI accounts for approx. 1% of all the PI species, and is present in both the nuclear and the plasma membrane. Remarkably, this C₂₆-PI is the only C₂₆-containing glycerophospholipid that is detectable in wild-type yeast, and the C₂₆-substitution is highly specific for the sn-1 position of the glycerol backbone. To characterize the biophysical properties of this lipid, it was chemically synthesized. In contrast to PIs with normal long-chain fatty acids (C₁₆ or C₁₈), the C₂₆-PI greatly reduced the bilayer to hexagonal phase transition of liposomes composed of 1,2-dielaidoyl-sn-glycero-3-phosphoethanolamine (DEPE). The biophysical properties of this lipid are thus consistent with a possible role in stabilizing highly curved membrane domains.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/299789
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