Optical imaging of the spatiotemporal dynamics of cerebral blood flow and oxidative metabolism in the rat barrel cortex
- Weber, B. Division of Nuclear Medicine, University Hospital Zurich, Switzerland - Max Planck Institute for Biological Cybernetics, Tübingen, Germany
- Burger, C. Division of Nuclear Medicine, University Hospital Zurich, Switzerland
- Wyss, M. T. Division of Nuclear Medicine, University Hospital Zurich, Switzerland
- Schulthess, G. K. von Division of Nuclear Medicine, University Hospital Zurich, Switzerland
- Scheffold, Frank Soft Condensed Matter Group, Department of Physics, University of Fribourg, Switzerland
- Buck, Alfred Division of Nuclear Medicine, University Hospital Zurich, Switzerland
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15.11.2004
Published in:
- European Journal of Neuroscience. - 2004, vol. 20, p. 2664-2670
English
Oxidative metabolism and cerebral blood flow (CBF) are two of the most important measures in neuroimaging. However, results from concurrent imaging of the two with high spatial and temporal resolution have never been published. We used flavoprotein autofluorescence (AF) and laser speckle imaging (LSI) in the anaesthetized rat to map oxidative metabolism and CBF in response to single vibrissa stimulation. Autofluorescence responses reflecting oxidative metabolism demonstrated a fast increase with a delay of 0.1 s. The sign-reversed speckle contrast reflecting CBF started to rise with a delay of 0.6 s and reached its maximum 1.4 s after the stimulation offset. The fractional signal changes were 2.0% in AF and 9.7% in LSI. Pixelwise modelling revealed that CBF maps spread over an area up to 2.5-times larger than metabolic maps. The results provide evidence that the increase in cerebral oxidative metabolism in response to sensory stimulation is considerably faster and more localized than the CBF response. This suggests that future developments in functional imaging concentrating on the metabolic response promise an increased spatial resolution.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Physique
- Language
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- English
- Classification
- Physics
- License
- License undefined
- Identifiers
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- RERO DOC 4199
- DOI 10.1111/j.1460-9568.2004.03735.x
- Persistent URL
- https://folia.unifr.ch/unifr/documents/299611
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