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Brain natriuretic peptide is able to stimulate cardiac progenitor cell proliferation and differentiation in murine hearts after birth

Bielmann, Christelle ; Rignault-Clerc, Stéphanie ; Liaudet, Lucas ; Li, Feng ; Kunieda, Tetsuo ; Sogawa, Chizuru ; Zehnder, Tamara ; Waeber, Bernard ; Feihl, François ; Rosenblatt-Velin, Nathalie

In: Basic Research in Cardiology, 2015, vol. 110, no. 1, p. 1-17

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    Titre
    • Brain natriuretic peptide is able to stimulate cardiac progenitor cell proliferation and differentiation in murine hearts after birth
    Auteur
    • Bielmann, Christelle. Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Bugnon 7a, 1005, Lausanne, Switzerland
    • Rignault-Clerc, Stéphanie. Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Bugnon 7a, 1005, Lausanne, Switzerland
    • Liaudet, Lucas. Service de Médecine Intensive Adulte, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland
    • Li, Feng. Department of Pathology and Laboratory Medecine, University of North Carolina, Chapel Hill, Wilmington, NC, USA
    • Kunieda, Tetsuo. Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan
    • Sogawa, Chizuru. Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan
    • Zehnder, Tamara. Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Bugnon 7a, 1005, Lausanne, Switzerland
    • Waeber, Bernard. Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Bugnon 7a, 1005, Lausanne, Switzerland
    • Feihl, François. Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Bugnon 7a, 1005, Lausanne, Switzerland
    • Rosenblatt-Velin, Nathalie. Division de Physiopathologie Clinique, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Bugnon 7a, 1005, Lausanne, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Basic Research in Cardiology, 2015, vol. 110, no. 1, p. 1-17. Springer Berlin Heidelberg
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:333028
    Summary
    Brain natriuretic peptide (BNP) contributes to heart formation during embryogenesis. After birth, despite a high number of studies aimed at understanding by which mechanism(s) BNP reduces myocardial ischemic injury in animal models, the actual role of this peptide in the heart remains elusive. In this study, we asked whether BNP treatment could modulate the proliferation of endogenous cardiac progenitor cells (CPCs) and/or their differentiation into cardiomyocytes. CPCs expressed the NPR-A and NPR-B receptors in neonatal and adult hearts, suggesting their ability to respond to BNP stimulation. BNP injection into neonatal and adult unmanipulated mice increased the number of newly formed cardiomyocytes (neonatal: +23%, p=0.009 and adult: +68%, p=0.0005) and the number of proliferating CPCs (neonatal: +142%, p=0.002 and adult: +134%, p=0.04). In vitro, BNP stimulated CPC proliferation via NPR-A and CPC differentiation into cardiomyocytes via NPR-B. Finally, as BNP might be used as a therapeutic agent, we injected BNP into mice undergoing myocardial infarction. In pathological conditions, BNP treatment was cardioprotective by increasing heart contractility and reducing cardiac remodelling. At the cellular level, BNP stimulates CPC proliferation in the non-infarcted area of the infarcted hearts. In the infarcted area, BNP modulates the fate of the endogenous CPCs but also of the infiltrating CD45+ cells. These results support for the first time a key role for BNP in controlling the progenitor cell proliferation and differentiation after birth. The administration of BNP might, therefore, be a useful component of therapeutic approaches aimed at inducing heart regeneration.