Faculté des sciences et de médecine
Supplementary material

KPC-50 confers resistance to ceftazidime-avibactam associated with reduced carbapenemase activity

Poirel, Laurent ; Vuillemin, Xavier ; Juhas, Mario ; Masseron, Amandine ; Bechtel-Grosch, Ursina ; Tiziani, Simon ; Mancini, Stefano ; Nordmann, Patrice

In: Antimicrobial Agents and Chemotherapy, 2020, vol. 64, no. 8, p. -

KPC-50 is a KPC-3 variant identified from a Klebsiella pneumoniae clinical isolate recovered in Switzerland in 2019. Compared to KPC-3, KPC-50 shows (i) a three- amino-acid insertion (Glu-Ala-Val) between amino acids 276 and 277, (ii) an increased affinity to ceftazidime, (iii) a decreased sensitivity to avibactam, explaining the ceftazidime-avibactam resistance, and (iv) an association with a... More

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    Title
    • KPC-50 confers resistance to ceftazidime-avibactam associated with reduced carbapenemase activity
    Author
    • Poirel, Laurent. Emerging Antibiotic Resistance Unit and Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland
    • Vuillemin, Xavier. Emerging Antibiotic Resistance Unit and Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland
    • Juhas, Mario. Emerging Antibiotic Resistance Unit and Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland
    • Masseron, Amandine. Emerging Antibiotic Resistance Unit and Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland
    • Bechtel-Grosch, Ursina. Department of Trauma Surgery, University Hospital of Zürich, Zürich, Switzerland
    • Tiziani, Simon. Institute of Intensive Medicine, University Hospital Zürich, University of Zürich, Zürich, Switzerland
    • Mancini, Stefano. Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland
    • Nordmann, Patrice. Emerging Antibiotic Resistance Unit and Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland
    Document Type
    • Postprint
    Publication Date
    • 2020-07-22
    Classification
    • Biology
    OAI-PMH Identifier
    • oai:doc.rero.ch:20201105105453-EV
    Submission No.
    • 20201105105453-EV
    Summary
    KPC-50 is a KPC-3 variant identified from a Klebsiella pneumoniae clinical isolate recovered in Switzerland in 2019. Compared to KPC-3, KPC-50 shows (i) a three- amino-acid insertion (Glu-Ala-Val) between amino acids 276 and 277, (ii) an increased affinity to ceftazidime, (iii) a decreased sensitivity to avibactam, explaining the ceftazidime-avibactam resistance, and (iv) an association with a sharp reduction of its carbapenemase activity.