Faculté des sciences et de médecine

Characterization of PAN-1, a carbapenem-hydrolyzing class b β-lactamase from the environmental gram-negative Pseudobacteriovorax antillogorgiicola

Kieffer, Nicolas ; Poirel, Laurent ; Fournier, Claudine ; Haltli, Brad ; Kerr, Russel ; Nordmann, Patrice

In: Frontiers in Microbiology, 2019, vol. 10, p. -

The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species Pseudobacteriovorax antillogorgiicola. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM- 1, its closest relative. Kinetic parameters performed on purified PAN-1 showed it displayed a hydrolytic activity toward most β-lactams including carbapenems but ... More

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    Summary
    The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species Pseudobacteriovorax antillogorgiicola. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM- 1, its closest relative. Kinetic parameters performed on purified PAN-1 showed it displayed a hydrolytic activity toward most β-lactams including carbapenems but spared cefepime and aztreonam. These results further highlight that environmental bacterial species may be reservoirs of MβL encoding genes.