Facoltà di scienze biomediche

Rationally modified estrogen receptor protein as a bio-recognition element for the detection of EDC pollutants : strategies and opportunities

Pedotti, Mattia ; Ferrero, Valentina Elisabetta Viviana ; Lettieri, Teresa ; Colpo, Pascal ; Follonier, Stephane ; Calzolai, Luigi ; Varani, Luca

In: International journal of environmental research and public health, 2015, vol. 12, no. 3, p. 2612-2621

The estrogen receptor protein (ER) can bind a vast number of organic pollutants widely spread in the environment and collectively known as Endocrine Disrupting Chemicals, EDCs. Its broad selectivity makes it an ideal bio-recognition element for the detection of EDCs. Here we describe the strategy and rationale for the design of ER based biosensors and assays that generate a signal in the... More

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    Summary
    The estrogen receptor protein (ER) can bind a vast number of organic pollutants widely spread in the environment and collectively known as Endocrine Disrupting Chemicals, EDCs. Its broad selectivity makes it an ideal bio-recognition element for the detection of EDCs. Here we describe the strategy and rationale for the design of ER based biosensors and assays that generate a signal in the presence of EDCs. The opportunity to use either natural or rationally modified ER molecules is discussed. The latter approach was successfully applied in the EU-FP7 project RADAR, with the aim to develop a novel biosensor for the detection of organic pollutants both in the environment and in commercial water products.