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Adipose-derived stem cells (ADSCs) and muscle precursor cells (MPCs) for the treatment of bladder voiding dysfunction

Tremp, Mathias ; Salemi, Souzan ; Largo, Remo ; Andersson, Karl-Erik ; A. Plock, Jan ; Aboushwareb, Tamer ; Sulser, Tullio ; Eberli, Daniel

In: World Journal of Urology, 2014, vol. 32, no. 5, p. 1241-1248

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    Titre
    • Adipose-derived stem cells (ADSCs) and muscle precursor cells (MPCs) for the treatment of bladder voiding dysfunction
    Auteur
    • Tremp, Mathias. Division of Urology, University Hospital Zürich (USZ), 8091, Zurich, Switzerland
    • Salemi, Souzan. Division of Urology, University Hospital Zürich (USZ), 8091, Zurich, Switzerland
    • Largo, Remo. Division of Urology, University Hospital Zürich (USZ), 8091, Zurich, Switzerland
    • Andersson, Karl-Erik. Wake Forest University Health Sciences, Wake Forest Institute for Regenerative Medicine, Medical Center Boulevard, 27154-1094, Winston Salem, NC, USA
    • A. Plock, Jan. Division of Plastic and Reconstructive Surgery, Department of Surgery, University Hospital Zürich, 8091, Zurich, Switzerland
    • Aboushwareb, Tamer. Wake Forest University Health Sciences, Wake Forest Institute for Regenerative Medicine, Medical Center Boulevard, 27154-1094, Winston Salem, NC, USA
    • Sulser, Tullio. Division of Urology, University Hospital Zürich (USZ), 8091, Zurich, Switzerland
    • Eberli, Daniel. Division of Urology, University Hospital Zürich (USZ), 8091, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • World Journal of Urology, 2014, vol. 32, no. 5, p. 1241-1248. Springer Berlin Heidelberg
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:325299
    Summary
    Purpose: Bladder outflow obstruction (BOO) is common in the elderly and can result in bladder voiding dysfunction (BVD) due to severe bladder muscle damage. The goal of this research was to evaluate the use of adult stem cells for the treatment of BVD due to decreased muscle contractility in a rat model. Materials and methods: Adipose-derived stem cells (ADSCs) and muscle precursor cells (MPCs) were harvested from male Lewis rats and expanded in culture. BOO was induced by tying a suture around the urethra. Six weeks after obstruction, the development of BVD was confirmed by cystometric analysis in conscious rats, histology and molecular investigations. Injection of ADSCs or MPCs into the bladder wall and synchronous deligation was performed 6weeks after the obstruction. After stem-cell treatment, morphological and functional changes were assessed. Age-matched rats and animals without cellular therapy but deligation-only served as controls. Results: Voiding pressures decreased progressively 6weeks after obstruction with increased bladder capacities. Structural changes of the detrusor muscle occurred during the time of obstruction with an increased connective tissue-to-smooth muscle ratio and decreased SMA/smoothelin expression. After stem-cell injection, improved voiding pressures and voiding volumes were observed together with recovered tissue architecture. RT-PCR and Western blotting showed an up-regulation of important contractile proteins. Conclusions: We established a reliable model for BVD and demonstrated that ADSCs and MPCs can prevent pathophysiological remodelling and provide regenerated bladder tissue and function.