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Tissue engineering of skin: human tonsil-derived mesenchymal cells can function as dermal fibroblasts

Böttcher-Haberzeth, Sophie ; Biedermann, Thomas ; Klar, Agnieszka ; Pontiggia, Luca ; Rac, Jürgen ; Nadal, David ; Schiestl, Clemens ; Reichmann, Ernst ; Meuli, Martin

In: Pediatric Surgery International, 2014, vol. 30, no. 2, p. 213-222

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    Titre
    • Tissue engineering of skin: human tonsil-derived mesenchymal cells can function as dermal fibroblasts
    Auteur
    • Böttcher-Haberzeth, Sophie. Tissue Biology Research Unit, University Children's Hospital Zurich, Zurich, Switzerland
    • Biedermann, Thomas. Tissue Biology Research Unit, University Children's Hospital Zurich, Zurich, Switzerland
    • Klar, Agnieszka. Tissue Biology Research Unit, University Children's Hospital Zurich, Zurich, Switzerland
    • Pontiggia, Luca. Tissue Biology Research Unit, University Children's Hospital Zurich, Zurich, Switzerland
    • Rac, Jürgen. Department of Infectious Diseases and Hospital Epidemiology, University Children's Hospital Zurich, Zurich, Switzerland
    • Nadal, David. Department of Infectious Diseases and Hospital Epidemiology, University Children's Hospital Zurich, Zurich, Switzerland
    • Schiestl, Clemens. Department of Surgery, University Children's Hospital Zurich, Zurich, Switzerland
    • Reichmann, Ernst. Tissue Biology Research Unit, University Children's Hospital Zurich, Zurich, Switzerland
    • Meuli, Martin. Department of Surgery, University Children's Hospital Zurich, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Pediatric Surgery International, 2014, vol. 30, no. 2, p. 213-222. Springer Berlin Heidelberg
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:325134
    Summary
    Purpose: It is unclear whether dermal fibroblasts are indispensable key players for tissue engineering of dermo-epidermal skin analogs. In this experimental study, we wanted to test the hypothesis that tonsil-derived mesenchymal cells can assume the role of dermal fibroblasts when culturing pigmented skin analogs for transplantation. Methods: Mesenchymal cells from excised tonsils and keratinocytes, melanocytes, and fibroblasts from skin biopsies were isolated, cultured, and expanded. Melanocytes and keratinocytes were seeded in a ratio of 1:5 onto collagen gels previously populated either with tonsil-derived mesenchymal cells or with autologous dermal fibroblasts. These laboratory engineered skin analogs were then transplanted onto full-thickness wounds of immuno-incompetent rats and analyzed after 3weeks with regard to macroscopic and microscopic epidermal characteristics. Results: The skin analogs containing tonsil-derived mesenchymal cells showed the same macroscopic appearance as the ones containing dermal fibroblasts. Histologically, features of epidermal stratification, pigmentation, and cornification were identical to those of the controls assembled with autologous dermal fibroblasts. Transmission electron microscopy confirmed these findings. Conclusion: These data suggest that human tonsil-derived mesenchymal cells can assume dermal fibroblast functions, indicating that possibly various types of mesenchymal cells can successfully be employed for "skingineering” purposes. This aspect may have clinical implications when sources for dermal fibroblasts are scarce.