Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release
- Venereau, Emilie Division of Genetics and Cell Biology, San Raffaele Scientific Institute, 20132 Milan, Italy
- Casalgrandi, Maura HMGBiotech Srl, 20133 Milan, Italy
- Schiraldi, Milena Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
- Antoine, Daniel J. Medical Research Council Centre for Drug Safety Science, Department of Molecular and Clinical Pharmacology, University of Liverpool, Liverpool L69 3BX, England, UK
- Cattaneo, Angela Division of Genetics and Cell Biology, San Raffaele Scientific Institute, 20132 Milan, Italy
- De Marchis, Francesco Division of Genetics and Cell Biology, San Raffaele Scientific Institute, 20132 Milan, Italy
- Liu, Jaron San Raffaele University, 20132 Milan, Italy
- Antonelli, Antonella San Raffaele University, 20132 Milan, Italy
- Preti, Alessandro HMGBiotech Srl, 20133 Milan, Italy
- Raeli, Lorenzo Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
- Samadi Shams, Sara San Raffaele University, 20132 Milan, Italy
- Yang, Huan Feinstein Institute for Medical Research, Manhasset, NY 11030
- Varani, Luca Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
- Andersson, Ulf Departments of Women’s and Children’s Health, Karolinska Institute and Karolinska University Hospital, SE-171 77 Stockholm, Sweden
- Tracey, Kevin J. Feinstein Institute for Medical Research, Manhasset, NY 11030
- Bachi, Angela Division of Genetics and Cell Biology, San Raffaele Scientific Institute, 20132 Milan, Italy
- Uguccioni, Mariagrazia Institute for Research in Biomedicine (IRB), Faculty of Biomedical Sciences, Università della Svizzera italiana, Switzerland
- Bianchi, Marco E. Division of Genetics and Cell Biology, San Raffaele Scientific Institute, 20132 Milan, Italy - San Raffaele University, 20132 Milan, Italy
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06.08.2012
Published in:
- The journal of experimental medicine. - 2012, vol. 209, no. 9, p. 1519-1528
English
Tissue damage causes inflammation, by recruiting leukocytes and activating them to release proinflammatory mediators. We show that high-mobility group box 1 protein (HMGB1) orchestrates both processes by switching among mutually exclusive redox states. Reduced cysteines make HMGB1 a chemoattractant, whereas a disulfide bond makes it a proinflammatory cytokine and further cysteine oxidation to sulfonates by reactive oxygen species abrogates both activities. We show that leukocyte recruitment and activation can be separated. A nonoxidizable HMGB1 mutant in which serines replace all cysteines (3S- HMGB1) does not promote cytokine production, but is more effective than wild-type HMGB1 in recruiting leukocytes in vivo. BoxA, a HMGB1 inhibitor, interferes with leukocyte recruitment but not with activation. We detected the different redox forms of HMGB1 ex vivo within injured muscle. HMGB1 is completely reduced at first and disulfide-bonded later. Thus, HMGB1 orchestrates both key events in sterile inflammation, leukocyte recruitment and their induction to secrete inflammatory cytokines, by adopting mutually exclusive redox states.
- Language
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- English
- Classification
- Medicine
- License
- License undefined
- Identifiers
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- RERO DOC 324428
- DOI 10.1084/jem.20120189
- ARK ark:/12658/srd1318895
- Persistent URL
- https://n2t.net/ark:/12658/srd1318895
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