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Hepatic lipid composition differs between ob/ob and ob/+ control mice as determined by using in vivo localized proton magnetic resonance spectroscopy

Ye, Qiong ; Danzer, Carsten ; Fuchs, Alexander ; Wolfrum, Christian ; Rudin, Markus

In: Magnetic Resonance Materials in Physics, Biology and Medicine, 2012, vol. 25, no. 5, p. 381-389

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    Titre
    • Hepatic lipid composition differs between ob/ob and ob/+ control mice as determined by using in vivo localized proton magnetic resonance spectroscopy
    Auteur
    • Ye, Qiong. Institute for Biomedical Engineering UZH/ETH, University and ETH Zurich, HIT E22.4, Wolfgang-Pauli-Strasse 27, 8093, Zurich, Switzerland
    • Danzer, Carsten. Institute of Cell Biology, ETH Zurich, Zurich, Switzerland
    • Fuchs, Alexander. Institute for Biomedical Engineering UZH/ETH, University and ETH Zurich, 8093, Zurich, Switzerland
    • Wolfrum, Christian. Laboratory of Translational Nutritional Biology, ETH Zurich, Zurich, Switzerland
    • Rudin, Markus. Institute for Biomedical Engineering UZH/ETH, University and ETH Zurich, HIT E22.4, Wolfgang-Pauli-Strasse 27, 8093, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Magnetic Resonance Materials in Physics, Biology and Medicine, 2012, vol. 25, no. 5, p. 381-389. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:322499
    Summary
    Object: Hepatic lipid accumulation is associated with nonalcoholic fatty liver disease, and the metabolic syndrome constitutes an increasing medical problem. In vivo proton magnetic resonance spectroscopy (1H MRS) allows the assessment of hepatic lipid levels noninvasively and also yields information on the fat composition due to its high spectral resolution. Materials and methods: We applied 1H MRS at 9.4T to study lipid content and composition in eight leptin-deficient ob/ob mice as a model of obesity and in four lean ob/+ control mice at 24weeks of age. PRESS sequence was used. For accurate estimation of signal intensity, differences in relaxation behavior of individual signals were accounted for each mouse individually. Also, in order to minimize spectral degrading due to motion artifacts, respiration gating was applied. Results: Significant differences between ob/ob and ob/+ control mice were found in both lipid content and composition. The mean chain length was found to be significantly longer in ob/ob mice with a higher fraction of monounsaturated lipids. Conclusion: 1H MRS enables accurate assessment in hepatic lipids in mice, which is attractive for mechanistic studies of altered metabolism given the large number of genetically engineered mouse models available