Fulltext

Diagonal chromatographic selection of cysteinyl peptides modified with benzoquinones

Dayon, Loïc ; Girault, Hubert

In: Analytical and Bioanalytical Chemistry, 2007, vol. 389, no. 3, p. 841-849

Zum persönliche Liste hinzufügen
    Titel
    • Diagonal chromatographic selection of cysteinyl peptides modified with benzoquinones
    Autor
    • Dayon, Loïc. Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 6, 1015, Lausanne, Switzerland
    • Girault, Hubert. Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 6, 1015, Lausanne, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Analytical and Bioanalytical Chemistry, 2007, vol. 389, no. 3, p. 841-849. Springer-Verlag
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:322122
    Summary
    The derivatization of cysteine-containing peptides with benzoquinone compounds is rapid, quantitative and specific in acidic media. The conversion of cysteines into hydrophobic benzoquinone-adducted residues in peptides is used here to alter the chromatographic properties of cysteinyl peptides during liquid chromatography separation. The benzoquinone derivatization is shown to allow the accurate selection of cysteine-containing peptides of bovine serum albumin tryptic digest by diagonal reversed-phase chromatography, which consists of one primary and a series of secondary identical liquid chromatographic separations, before and after a cysteinyl-targeted modification of the peptides by benzoquinone compounds. Figure Diagonal chromatographic selection of cysteinyl peptides modified with benzoquinones