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Autonomous microfluidic multi-channel chip for real-time PCR with integrated liquid handling

Frey, Olivier ; Bonneick, Sonja ; Hierlemann, Andreas ; Lichtenberg, Jan

In: Biomedical Microdevices, 2007, vol. 9, no. 5, p. 711-718

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    Titre
    • Autonomous microfluidic multi-channel chip for real-time PCR with integrated liquid handling
    Auteur
    • Frey, Olivier. Sensors, Actuators and Microsystems Laboratory, Institute of Microtechnology, University of Neuchâtel, Rue Jaquet-Droz 1, 2000, Neuchâtel, Switzerland
    • Bonneick, Sonja. Physical Electronics Laboratory, ETH Zurich, Wolfgang-Pauli-Str. 16, 8093, Zurich, Switzerland
    • Hierlemann, Andreas. Physical Electronics Laboratory, ETH Zurich, Wolfgang-Pauli-Str. 16, 8093, Zurich, Switzerland
    • Lichtenberg, Jan. Physical Electronics Laboratory, ETH Zurich, Wolfgang-Pauli-Str. 16, 8093, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Biomedical Microdevices, 2007, vol. 9, no. 5, p. 711-718. Springer US; http://www.springer-ny.com
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:320476
    Summary
    We report on a novel, polymer-based, multi-channel device for polymerase chain reaction that combines, for the first time, rapid sample processing in less than 5min with high throughput at low costs. This is achieved by sample shuttling, during which submicroliter sample plugs (∼100nl) are oscillated rapidly over three constant-temperature zones by pneumatic actuation with integrated system. The accuracy and the speed of the liquid handling have been significantly increased, while the design of the device can be kept very simple and allows for mass production using conventional low-cost polymer fabrication processes. Massive parallelization can lead to a throughput up to 100 samples in 10min including the preparation time. The amplification can be optically monitored by means of online fluorescence detection. Successful real-time PCR and the determination of the threshold cycle, C t, using the developed device were demonstrated with plasmid DNA in a fluorescent real-time format