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Accurate measurement of residual dipolar couplings in anisotropic phase

Cutting, Brian ; Tolman, Joel ; Nanchen, Steve ; Bodenhausen, Geoffrey

In: Journal of Biomolecular NMR, 2002, vol. 23, no. 3, p. 195-200

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    Title
    • Accurate measurement of residual dipolar couplings in anisotropic phase
    Author
    • Cutting, Brian. Institut de Chimie Moléculaire et Biologique, Ecole Polytechnique Fédérale de Lausanne, BCH, 1015, Lausanne, Switzerland
    • Tolman, Joel. Institut de Chimie Moléculaire et Biologique, Ecole Polytechnique Fédérale de Lausanne, BCH, 1015, Lausanne, Switzerland
    • Nanchen, Steve. Institut de Chimie Moléculaire et Biologique, Ecole Polytechnique Fédérale de Lausanne, BCH, 1015, Lausanne, Switzerland
    • Bodenhausen, Geoffrey. Institut de Chimie Moléculaire et Biologique, Ecole Polytechnique Fédérale de Lausanne, BCH, 1015, Lausanne, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Journal of Biomolecular NMR, 2002, vol. 23, no. 3, p. 195-200. Kluwer Academic Publishers
    Other Version
    OAI-PMH Identifier
    • oai:doc.rero.ch:320361
    Summary
    The determination of residual dipolar couplings (RDCs) by quantitative J spectroscopy methods such as Heteronuclear Single Quantum Correlation with Phase Encoded Coupling (HSQC-PEC) is prone to systematic errors that may be caused by differential attenuation during the conversion of orthogonal density operator components into observable terms. The attenuation may be caused by miscalibration of radio-frequency pulses and by relaxation effects. A simple method is presented that allows one to remove most of these systematic errors without losses in sensitivity or resolution