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Chronic nicotine treatment impacts the regulation of opioid and non-opioid peptides in the rat dorsal striatum

  • Petruzziello, Filomena Visual Cognition Laboratory, Department of Medicine, University of Fribourg, Switzerland
  • Falasca, Sara Visual Cognition Laboratory, Department of Medicine, University of Fribourg, Switzerland
  • Andren, Per E. Department of Pharmaceutical Biosciences, Medical Mass Spectrometry, Uppsala University, Sweden
  • Rainer, Gregor Visual Cognition Laboratory, Department of Medicine, University of Fribourg, Switzerland
  • Zhang, Xiaozhe Visual Cognition Laboratory, Department of Medicine, University of Fribourg, Switzerland
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    22.02.2013
Published in:
  • Molecular and Cellular Proteomics. - 2013, vol. 12, no. 6, p. 1553-1562
Neurobiology
Peptides
Protein Sequence
Sequencing MS
Tandem Mass Spectrometry
English The chronic use of nicotine, the main psychoactive ingredient of tobacco smoking, alters diverse physiological processes and consequently generates physical dependence. To understand the impact of chronic nicotine on neuropeptides, which are potential molecules associated with dependence, we conducted qualitative and quantitative neuropeptidomics on the rat dorsal striatum (DS), an important brain region implicated in the preoccupation/craving phase of drug dependence. We used extensive LC-FT-MS/MS analyses for neuropeptide identification and LC-FT-MS in conjunction with stable isotope addition for relative quantification. The treatment with chronic nicotine for three months led to moderate changes in the levels of endogenous DS peptides. Five enkephalin opioid peptides were up-regulated, while no change was observed for dynorphin peptides. Specially, nicotine altered levels of 9 non-opioid peptides derived from precursors including somatostatin and cerebellin, which potentially modulate neurotransmitter release and energy metabolism. This broad but selective impact on the multiple peptidergic systems suggests that apart from the opioid peptides, several other peptidergic systems are involved in the preoccupation/craving phase of drug dependence. Our finding permits future evaluation of the neurochemical circuits modulated by chronic nicotine exposure and provides a number of novel molecules that could serve as potential therapeutic targets for treating drug dependence.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/303013
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