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Isolation and characterization of major histocompatibility complex (MHC) class II B genes in the Barn owl (Aves: Tyto alba )

Burri, Reto ; Niculita-Hirzel, Hélène ; Roulin, Alexandre ; Fumagalli, Luca

In: Immunogenetics, 2008, vol. 60, no. 9, p. 543-550

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    Titre
    • Isolation and characterization of major histocompatibility complex (MHC) class II B genes in the Barn owl (Aves: Tyto alba )
    Auteur
    • Burri, Reto. Laboratory for Conservation Biology, Department of Ecology and Evolution, Biophore, University of Lausanne, 1015, Lausanne, Switzerland
    • Niculita-Hirzel, Hélène. Department of Ecology and Evolution, Biophore, University of Lausanne, 1015, Lausanne, Switzerland
    • Roulin, Alexandre. Department of Ecology and Evolution, Biophore, University of Lausanne, 1015, Lausanne, Switzerland
    • Fumagalli, Luca. Laboratory for Conservation Biology, Department of Ecology and Evolution, Biophore, University of Lausanne, 1015, Lausanne, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:318958
    Summary
    We isolated major histocompatibility complex class II B (MHCIIB) genes in the Barn owl (Tyto alba). A PCR-based approach combined with primer walking on genomic and complementary DNA as well as Southern blot analyses revealed the presence of two MHCIIB genes, both being expressed in spleen, liver, and blood. Characteristic structural features of MHCIIB genes as well as their expression and high non-synonymous substitution rates in the region involved in antigen binding suggest that both genes are functional. MHC organization in the Barn owl is simple compared to passerine species that show multiple duplications, and resembles the minimal essential MHC of chicken