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If You Don't Look, You Won't See: Intravital Multiphoton Imaging of Primary and Metastatic Breast Cancer

Bonapace, Laura ; Wyckoff, Jeffrey ; Oertner, Thomas ; Van Rheenen, Jacco ; Junt, Tobias ; Bentires-Alj, Mohamed

In: Journal of Mammary Gland Biology and Neoplasia, 2012, vol. 17, no. 2, p. 125-129

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    Titre
    • If You Don't Look, You Won't See: Intravital Multiphoton Imaging of Primary and Metastatic Breast Cancer
    Auteur
    • Bonapace, Laura. Friedrich Miescher Institute for Biomedical Research (FMI), Maulbeerstr. 66, 4058, Basel, Switzerland
    • Wyckoff, Jeffrey. Friedrich Miescher Institute for Biomedical Research (FMI), Maulbeerstr. 66, 4058, Basel, Switzerland
    • Oertner, Thomas. Friedrich Miescher Institute for Biomedical Research (FMI), Maulbeerstr. 66, 4058, Basel, Switzerland
    • Van Rheenen, Jacco. Hubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, The Netherlands
    • Junt, Tobias. Novartis Institutes for Biomedical Research, Basel, Switzerland
    • Bentires-Alj, Mohamed. Friedrich Miescher Institute for Biomedical Research (FMI), Maulbeerstr. 66, 4058, Basel, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of Mammary Gland Biology and Neoplasia, 2012, vol. 17, no. 2, p. 125-129. Springer US; http://www.springer-ny.com
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:318175
    Summary
    A fundamental hallmark of cancer is progression to metastasis and the growth of breast cancer metastases in lung, bone, liver and/or brain causes fatal complications. Unfortunately, the cellular and biochemical mechanisms of the metastatic process remain ill-defined. Recent application of intravital multiphoton microscopy (MP-IVM) to image fluorescently labeled cells in mouse models of cancer has allowed dynamic observation of this multi-step process at the cellular and subcellular levels. In this article, we discuss the use of MP-IVM in studies of breast cancer metastasis, as well as surgical techniques for exposing tumors prior to imaging. We also describe a versatile multiphoton microscope for imaging tumor-stroma interactions