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Bubble cell for magnetic bead trapping in capillary electrophoresis

Gassner, Anne-Laure ; Proczek, Gaëlle ; Girault, Hubert

In: Analytical and Bioanalytical Chemistry, 2011, vol. 401, no. 10, p. 3239-3248

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    Titel
    • Bubble cell for magnetic bead trapping in capillary electrophoresis
    Autor
    • Gassner, Anne-Laure. Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, EPFL SB ISIC LEPA, Station 6, 1015, Lausanne, Switzerland
    • Proczek, Gaëlle. Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, EPFL SB ISIC LEPA, Station 6, 1015, Lausanne, Switzerland
    • Girault, Hubert. Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, EPFL SB ISIC LEPA, Station 6, 1015, Lausanne, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Analytical and Bioanalytical Chemistry, 2011, vol. 401, no. 10, p. 3239-3248. Springer-Verlag
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:317384
    Summary
    A bubble cell capillary classically used to extend the optical path length for UV-vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case with magnetic beads trapped in a standard capillary, thereby having less effect on the experimental conditions. Using numerical simulations and microscopic visualizations, the capture of beads inside a bubble cell was investigated with two magnet configurations. Pressure-driven and electro-osmotic flow velocities were measured for different amounts of protein-A-coated beads or C18-functionalized beads (RPC-18). Solid-phase extraction of a model antibody on protein-A beads and preconcentration of fluorescein on RPC-18 beads were performed as proof of concept experiments. Figure Isovalues of the magnetic induction produced by two permanent magnets in attraction configuration with a capillary placed between them