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Apoptotic cell death in the lactating mammary gland is enhanced by a folding variant of α-lactalbumin

Baltzer, A. ; Svanborg, C. ; Jaggi, R.

In: Cellular and Molecular Life Sciences CMLS, 2004, vol. 61, no. 10, p. 1221-1228

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    Titre
    • Apoptotic cell death in the lactating mammary gland is enhanced by a folding variant of α-lactalbumin
    Auteur
    • Baltzer, A.. Department of Clinical Research, University of Bern, Murtenstrasse 35, 3010, Bern, Switzerland
    • Svanborg, C.. Division of Microbiology, Immunology and Glycobiology, Lund University, 223 62, Lund, Sweden
    • Jaggi, R.. Department of Clinical Research, University of Bern, Murtenstrasse 35, 3010, Bern, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Cellular and Molecular Life Sciences CMLS, 2004, vol. 61, no. 10, p. 1221-1228. Birkhäuser-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:317004
    Summary
    Apoptosis is essential to eliminate secretory epithelial cells during the involution of the mammary gland. The environmental regulation of this process is however, poorly understood. This study tested the effect of HAMLET (human α-lactalbumin made lethal to tumor cells) on mammary cells. Plastic pellets containing HAMLET were implanted into the fourth inguinal mammary gland of lactating mice for 3 days. Exposure of mammary tissue to HAMLET resulted in morphological changes typical for apoptosis and in a stimulation of caspase-3 activity in alveolar epithelial cells near the HAMLET pellets but not more distant to the pellet or in contralateral glands. The effect was specific for HAMLET and no effects were observed when mammary glands were exposed to native a-lactalbumin or fatty acid alone. HAMLET also induced cell death in vitro in a mouse mammary epithelial cell line. The results suggest that HAMLET can mediate apoptotic cell death in mammary gland tissue