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Benzoate-driven dehalogenation of chlorinated ethenes in microbial cultures from a contaminated aquifer

Bunge, Michael ; Kleikemper, Jutta ; Miniaci, Ciro ; Duc, Laurence ; Muusse, Margje ; Hause, Gerd ; Zeyer, Josef

In: Applied Microbiology and Biotechnology, 2007, vol. 76, no. 6, p. 1447-1456

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    Titre
    • Benzoate-driven dehalogenation of chlorinated ethenes in microbial cultures from a contaminated aquifer
    Auteur
    • Bunge, Michael. Soil Biology, Institute of Biogeochemistry and Pollutant Dynamics, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland
    • Kleikemper, Jutta. Soil Biology, Institute of Biogeochemistry and Pollutant Dynamics, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland
    • Miniaci, Ciro. Soil Biology, Institute of Biogeochemistry and Pollutant Dynamics, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland
    • Duc, Laurence. Soil Biology, Institute of Biogeochemistry and Pollutant Dynamics, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland
    • Muusse, Margje. Soil Biology, Institute of Biogeochemistry and Pollutant Dynamics, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland
    • Hause, Gerd. Biocenter, Martin Luther University of Halle-Wittenberg, Halle (Saale), Germany
    • Zeyer, Josef. Soil Biology, Institute of Biogeochemistry and Pollutant Dynamics, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Applied Microbiology and Biotechnology, 2007, vol. 76, no. 6, p. 1447-1456. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:316575
    Summary
    Microbial dehalogenation of tetrachloroethene (PCE) and cis-dichloroethene (cis-DCE) was studied in cultures from a continuous stirred tank reactor initially inoculated with aquifer material from a PCE-contaminated site. Cultures amended with hydrogen and acetate readily dechlorinated PCE and cis-DCE; however, this transformation was incomplete and resulted in the accumulation of chlorinated intermediates and only small amounts of ethene within 60days of incubation. Conversely, microbial PCE and cis-DCE dechlorination in cultures with benzoate and acetate resulted in the complete transformation to ethene within 30days. Community fingerprinting by denaturing gradient gel electrophoresis (DGGE) revealed the predominance of phylotypes closely affiliated with Desulfitobacterium, Dehalococcoides, and Syntrophus species. The Dehalococcoides culture VZ, obtained from small whitish colonies in cis-DCE dechlorinating agarose cultures, revealed an irregular cell diameter between 200 and 500nm, and a spherical or biconcave disk-shaped morphology. These organisms were identified as responsible for the dechlorination of cis-DCE to ethene in the PCE-dechlorinating consortia, operating together with the Desulfitobacterium as PCE-to-cis-DCE dehalogenating bacterium and with a Syntrophus species as potential hydrogen-producing partner in cultures with benzoate