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Analysis of epidermis- and mesophyll-specific transcript accumulation in powdery mildew-inoculated wheat leaves

Bruggmann, Rémy ; Abderhalden, Olaf ; Reymond, Philippe ; Dudler, Robert

In: Plant Molecular Biology, 2005, vol. 58, no. 2, p. 247-267

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    Title
    • Analysis of epidermis- and mesophyll-specific transcript accumulation in powdery mildew-inoculated wheat leaves
    Author
    • Bruggmann, Rémy. Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zürich, Switzerland
    • Abderhalden, Olaf. Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zürich, Switzerland
    • Reymond, Philippe. Department of Plant Molecular Biology, University of Lausanne, Biology Building, CH-1015, Lausanne, Switzerland
    • Dudler, Robert. Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8000, Zürich, Switzerland
    Document Type
    • Postprint
    OAI-PMH Identifier
    • oai:doc.rero.ch:315502
    Summary
    Powdery mildew is an important disease of wheat caused by the obligate biotrophic fungus Blumeria graminis f. sp. tritici. This pathogen invades exclusively epidermal cells after penetrating directly through the cell wall. Because powdery mildew colonizes exclusively epidermal cells, it is of importance not only to identify genes which are activated, but also to monitor tissue specificity of gene activation. Acquired resistance of wheat to powdery mildew can be induced by a previous inoculation with the non-host pathogen B. graminis f. sp. hordei, the causal agent of barley powdery mildew. The establishment of the resistant state is accompanied by the activation of genes. Here we report the tissue-specific cDNA-AFLP analysis and cloning of transcripts accumulating 6 and 24 h after the resistance-inducing inoculation with B. graminis f. sp. hordei. A total of 25 000 fragments estimated to represent about 17 000 transcripts were displayed. Out of these, 141 transcripts, were found to accumulate after Bgh inoculation using microarray hybridization analysis. Forty-four accumulated predominantly in the epidermis whereas 76 transcripts accumulated mostly in mesophyll tissue